Exploring the therapeutic potential of an antinociceptive and anti-inflammatory peptide from wasp venom.

Autor: Galante P; Laboratory of Neuropharmacology, Department of Physiological Sciences, University of Brasília, Brasília, DF, 70910-900, Brazil., Campos GAA; Laboratory of Neuropharmacology, Department of Physiological Sciences, University of Brasília, Brasília, DF, 70910-900, Brazil., Moser JCG; Laboratory of Neuropharmacology, Department of Physiological Sciences, University of Brasília, Brasília, DF, 70910-900, Brazil., Martins DB; Department of Physics, IBILCE, São Paulo State University, São José do Rio Preto, SP, 15054-000, Brazil., Dos Santos Cabrera MP; Department of Physics, IBILCE, São Paulo State University, São José do Rio Preto, SP, 15054-000, Brazil., Rangel M; Immunopathology Laboratory, Butantan Institute, Sao Paulo, SP, 05503-900, Brazil., Coelho LC; Laboratory of Applied Immunology, Department of Cell Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., Simon KS; Laboratory of Applied Immunology, Department of Cell Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., Amado VM; Faculty of Medicine and University Hospital of Brasília, University of Brasilia, Brasilia, DF, 79910-900, Brazil., de A I Muller J; Laboratory of Pharmacology and Inflammation FACFAN, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, 79070-900, Brazil., Koehbach J; Institute for Molecular Bioscience, Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, QLD, 4072, Australia., Lohman RJ; School of Pharmacy, The University of Queensland, Brisbane, QLD, 4072, Australia., Cabot PJ; School of Pharmacy, The University of Queensland, Brisbane, QLD, 4072, Australia., Vetter I; Institute for Molecular Bioscience, Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, QLD, 4072, Australia., Craik DJ; Institute for Molecular Bioscience, Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, QLD, 4072, Australia., Toffoli-Kadri MC; Laboratory of Pharmacology and Inflammation FACFAN, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, 79070-900, Brazil., Monge-Fuentes V; Laboratory of Neuropharmacology, Department of Physiological Sciences, University of Brasília, Brasília, DF, 70910-900, Brazil., Goulart JT; Laboratory of Neuropharmacology, Department of Physiological Sciences, University of Brasília, Brasília, DF, 70910-900, Brazil., Schwartz EF; Laboratory of Neuropharmacology, Department of Physiological Sciences, University of Brasília, Brasília, DF, 70910-900, Brazil., Silva LP; Laboratory of Nanobiotechnology, Embrapa Genetic Resources and Biotechnology, Brasília, DF, 70770917, Brazil., Bocca AL; Laboratory of Applied Immunology, Department of Cell Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., Mortari MR; Laboratory of Neuropharmacology, Department of Physiological Sciences, University of Brasília, Brasília, DF, 70910-900, Brazil. mmortari@unb.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Aug 01; Vol. 13 (1), pp. 12491. Date of Electronic Publication: 2023 Aug 01.
DOI: 10.1038/s41598-023-38828-w
Abstrakt: Animal venoms are rich sources of neuroactive compounds, including anti-inflammatory, antiepileptic, and antinociceptive molecules. Our study identified a protonectin peptide from the wasp Parachartergus fraternus' venom using mass spectrometry and cDNA library construction. Using this peptide as a template, we designed a new peptide, protonectin-F, which exhibited higher antinociceptive activity and less motor impairment compared to protonectin. In drug interaction experiments with naloxone and AM251, Protonectin-F's activity was decreased by opioid and cannabinoid antagonism, two critical antinociception pathways. Further experiments revealed that this effect is most likely not induced by direct action on receptors but by activation of the descending pain control pathway. We noted that protonectin-F induced less tolerance in mice after repeated administration than morphine. Protonectin-F was also able to decrease TNF-α production in vitro and modulate the inflammatory response, which can further contribute to its antinociceptive activity. These findings suggest that protonectin-F may be a potential molecule for developing drugs to treat pain disorders with fewer adverse effects. Our results reinforce the biotechnological importance of animal venom for developing new molecules of clinical interest.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje