Nano solid phase micro membrane tip and electrochemical methods for vanillin analysis in chocolate samples.

Autor: Ali I; Department of Chemistry, Jamia Millia Islamia (Central University), Jamia Nagar, New Delhi, 110025, India. Electronic address: drimran.chiral@gmail.com., Mısır M; Kırşehir Ahi Evran University, Faculty of Engineering and Architecture, 40100, Kırşehir, Turkey., Demir E; Afyonkarahisar Health Sciences University, Faculty of Pharmacy, Department of Analytical Chemistry, 03030, Afyonkarahisar, Turkey., Dinçer İ; Afyonkarahisar Health Sciences University, Faculty of Pharmacy, Department of Analytical Chemistry, 03030, Afyonkarahisar, Turkey., Locatelli M; Department of Pharmacy, University of Chieti-Pescara 'G. d'Annunzio ', Via dei Vestini 31, Chieti, 66100, Italy., ALOthman ZA; Department of Chemistry, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2023 Sep 15; Vol. 677, pp. 115268. Date of Electronic Publication: 2023 Jul 29.
DOI: 10.1016/j.ab.2023.115268
Abstrakt: A polymer-based nanosensor and electrochemical methods were developed for the quantitative analysis of vanillin. The sample preparation was done using nano solid phase micro membrane tip extraction (NSPMMTE). A novel poly(phenylalanine)/TiO 2 /CPE sensor was built as the working electrode for the first time for the analysis of the vanillin substance. The electrochemical behavior and analytical performance of vanillin were examined in detail by cyclic voltammetry (CV) and differential pulse stripping voltammetry (DPSV) techniques via the oxidation process. The optimized modules of the DPSV technique that affected the vanillin peak current and peak potential were pH, pulse amplitude, step potential, and deposition time. The electroactive surface areas of bare CPE, TiO 2 /CPE, and poly(phenylalanine)/TiO 2 /CPE electrodes were found to be 0.135 cm 2 , 0.155 cm 2 , and 0.221 cm 2 , respectively. The limit of detection (LOD) was 32.6 μg/L in the 0.25-15.0 mg/L working range at pH 7.0. The selectivity of the proposed DPSV method for the determination of vanillin on the modified electrode was investigated in the presence of various organic and inorganic substances, and the determination of vanillin with high recovery was achieved with less than 5% relative error. The analytical application was applied in chocolate samples and the DPSV method was found highly efficient, reproducible, and selective.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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