Enzyme immobilization technology as a tool to innovate in the production of biofuels: A special review of the Cross-Linked Enzyme Aggregates (CLEAs) strategy.

Autor: Costa IO; Departamento de Engenharia Química, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil., Morais JRF; Departamento de Engenharia Química, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil., de Medeiros Dantas JM; Departamento de Engenharia Química, McGill University, Montréal, QC, Canada., Gonçalves LRB; Departamento de Engenharia Química, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Dos Santos ES; Departamento de Engenharia Química, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil. Electronic address: everaldo.santos@ufrn.br., Rios NS; Departamento de Engenharia Química, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil. Electronic address: nathalia.rios@ufrn.br.
Jazyk: angličtina
Zdroj: Enzyme and microbial technology [Enzyme Microb Technol] 2023 Oct; Vol. 170, pp. 110300. Date of Electronic Publication: 2023 Jul 26.
DOI: 10.1016/j.enzmictec.2023.110300
Abstrakt: This review emphasizes the crucial role of enzyme immobilization technology in advancing the production of two main biofuels, ethanol and biodiesel, with a specific focus on the Cross-linked Enzyme Aggregates (CLEAs) strategy. This method of immobilization has gained attention due to its simplicity and affordability, as it does not initially require a solid support. CLEAs synthesis protocol includes two steps: enzyme precipitation and cross-linking of aggregates using bifunctional agents. We conducted a thorough search for papers detailing the synthesis of CLEAs utilizing amylases, cellulases, and hemicellulases. These key enzymes are involved in breaking down starch or lignocellulosic materials to produce ethanol, both in first and second-generation processes. CLEAs of lipases were included as these enzymes play a crucial role in the enzymatic process of biodiesel production. However, when dealing with large or diverse substrates such as lignocellulosic materials for ethanol production and oils/fats for biodiesel production, the use of individual enzymes may not be the most efficient method. Instead, a system that utilizes a blend of enzymes may prove to be more effective. To innovate in the production of biofuels (ethanol and biodiesel), enzyme co-immobilization using different enzyme species to produce Combi-CLEAs is a promising trend.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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