Identification of an autotransporter peptidase of Rickettsia rickettsii responsible for maturation of surface exposed autotransporters.

Autor: Nock AM; Host-Parasite Interactions Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, NIAID, NIH; Hamilton, Montana, United States of America., Aistleitner K; Host-Parasite Interactions Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, NIAID, NIH; Hamilton, Montana, United States of America., Clark TR; Host-Parasite Interactions Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, NIAID, NIH; Hamilton, Montana, United States of America., Sturdevant D; Genomics Research Section, Research Technologies Branch, Rocky Mountain Laboratories, NIAID, NIH; Hamilton, Montana, United States of America., Ricklefs S; Genomics Research Section, Research Technologies Branch, Rocky Mountain Laboratories, NIAID, NIH; Hamilton, Montana, United States of America., Virtaneva K; Genomics Research Section, Research Technologies Branch, Rocky Mountain Laboratories, NIAID, NIH; Hamilton, Montana, United States of America., Zhang Y; Protein Chemistry Unit, Research Technologies Branch, Rocky Mountain Laboratories, NIAID, NIH; Hamilton, Montana, United States of America., Gulzar N; Integrated Data Sciences Section, Research Technologies Branch, NIAID, NIH, Bethesda, Maryland, United States of America., Redekar N; Integrated Data Sciences Section, Research Technologies Branch, NIAID, NIH, Bethesda, Maryland, United States of America., Roy A; Bioinformatics and Computational Biology Branch, Office of Cyber Infrastructure and Computational Biology, Rocky Mountain Laboratories, NIAID, NIH; Hamilton, Montana, United States of America., Hackstadt T; Host-Parasite Interactions Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, NIAID, NIH; Hamilton, Montana, United States of America.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2023 Jul 31; Vol. 19 (7), pp. e1011527. Date of Electronic Publication: 2023 Jul 31 (Print Publication: 2023).
DOI: 10.1371/journal.ppat.1011527
Abstrakt: Members of the spotted fever group rickettsia express four large, surface-exposed autotransporters, at least one of which is a known virulence determinant. Autotransporter translocation to the bacterial outer surface, also known as type V secretion, involves formation of a β-barrel autotransporter domain in the periplasm that inserts into the outer membrane to form a pore through which the N-terminal passenger domain is passed and exposed on the outer surface. Two major surface antigens of Rickettsia rickettsii, are known to be surface exposed and the passenger domain cleaved from the autotransporter domain. A highly passaged strain of R. rickettsii, Iowa, fails to cleave these autotransporters and is avirulent. We have identified a putative peptidase, truncated in the Iowa strain, that when reconstituted into Iowa restores appropriate processing of the autotransporters as well as restoring a modest degree of virulence.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.)
Databáze: MEDLINE
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