Collagen membrane functionalized with magnesium oxide via room-temperature atomic layer deposition promotes osteopromotive and antimicrobial properties.

Autor:	Saha S; Department of Biomedical Engineering, University of Illinois Chicago, Chicago, USA., Costa RC; Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil., Silva MC; Department of Diagnosis and Surgery, Division of Oral and Maxillofacial Surgery and Implantology, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil., Fonseca-Santos JM; Department of Diagnosis and Surgery, Division of Oral and Maxillofacial Surgery and Implantology, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil., Chen L; Department of Periodontics, Center for Wound Healing and Tissue Regeneration, College of Dentistry, University of Illinois Chicago, Chicago, USA., Phakatkar AH; Department of Biomedical Engineering, University of Illinois Chicago, Chicago, USA., Bhatia H; Department of Chemical Engineering, University of Illinois Chicago, Chicago, USA., Faverani LP; Department of Diagnosis and Surgery, Division of Oral and Maxillofacial Surgery and Implantology, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil., Barão VAR; Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil., Shokuhfar T; Department of Biomedical Engineering, University of Illinois Chicago, Chicago, USA., Sukotjo C; Department of Restorative Dentistry, University of Illinois Chicago College of Dentistry, Chicago, USA., Takoudis C; Department of Biomedical Engineering, University of Illinois Chicago, Chicago, USA.; Department of Chemical Engineering, University of Illinois Chicago, Chicago, USA.
Jazyk:	angličtina
Zdroj:	Bioactive materials [Bioact Mater] 2023 Jul 24; Vol. 30, pp. 46-61. Date of Electronic Publication: 2023 Jul 24 (Print Publication: 2023).
DOI:	10.1016/j.bioactmat.2023.07.013
Abstrakt:	Artificial bone grafting materials such as collagen are gaining interest due to the ease of production and implantation. However, collagen must be supplemented with additional coating materials for improved osteointegration. Here, we report room-temperature atomic layer deposition (ALD) of MgO, a novel method to coat collagen membranes with MgO. Characterization techniques such as X-ray photoelectron spectroscopy, Raman spectroscopy, and electron beam dispersion mapping confirm the chemical nature of the film. Scanning electron and atomic force microscopies show the surface topography and morphology of the collagen fibers were not altered during the ALD of MgO. Slow release of magnesium ions promotes bone growth, and we show the deposited MgO film leaches trace amounts of Mg when incubated in phosphate-buffered saline at 37 °C. The coated collagen membrane had a superhydrophilic surface immediately after the deposition of MgO. The film was not toxic to human cells and demonstrated antibacterial properties against bacterial biofilms. Furthermore, in vivo studies performed on calvaria rats showed MgO-coated membranes (200 and 500 ALD) elicit a higher inflammatory response, leading to an increase in angiogenesis and a greater bone formation, mainly for Col-MgO500, compared to uncoated collagen. Based on the characterization of the MgO film and in vitro and in vivo data, the MgO-coated collagen membranes are excellent candidates for guided bone regeneration. Competing Interests: The authors declare that they have no competing financial interests or personal relationships that could have appeared to influence the work reported in this manuscript. (© 2023 The Authors.)
Databáze:	MEDLINE
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