Identification of a new DC-SIGN binding pentamannoside epitope within the complex structure of Candida albicans mannan.

Autor: Krylov VB; Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia., Gómez-Redondo M; CIC bioGUNE, Basque Research Technology Alliance, BRTA, 48160 Derio, Spain., Solovev AS; Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia., Yashunsky DV; Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia., Brown AJP; Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter EX4 4QD, United Kingdom., Stappers MHT; Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter EX4 4QD, United Kingdom., Gow NAR; Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter EX4 4QD, United Kingdom., Ardá A; CIC bioGUNE, Basque Research Technology Alliance, BRTA, 48160 Derio, Spain., Jiménez-Barbero J; CIC bioGUNE, Basque Research Technology Alliance, BRTA, 48160 Derio, Spain.; IKERBASQUE, Basque Foundation for Science and Technology, Euskadi Plaza 5, 48009 Bilbao, Spain.; Department of Organic & Inorganic Chemistry, Faculty of Science and Technology, University of the Basque Country, 48940 Leioa, Spain.; Centro de Investigacion Biomedica En Red de Enfermedades Respiratorias, Madrid, Spain., Nifantiev NE; Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Jazyk: angličtina
Zdroj: Cell surface (Amsterdam, Netherlands) [Cell Surf] 2023 Jul 22; Vol. 10, pp. 100109. Date of Electronic Publication: 2023 Jul 22 (Print Publication: 2023).
DOI: 10.1016/j.tcsw.2023.100109
Abstrakt: The dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) is an innate immune C-type lectin receptor that recognizes carbohydrate-based pathogen associated with molecular patterns of various bacteria, fungi, viruses and protozoa. Although a range of highly mannosylated glycoproteins have been shown to induce signaling via DC-SIGN, precise structure of the recognized oligosaccharide epitope is still unclear. Using the array of oligosaccharides related to selected fragments of main fungal antigenic polysaccharides we revealed a highly specific pentamannoside ligand of DC-SIGN, consisting of α-(1 → 2)-linked mannose chains with one inner α-(1 → 3)-linked unit. This structural motif is present in Candida albicans cell wall mannan and corresponds to its antigenic factors 4 and 13b. This epitope is not ubiquitous in other yeast species and may account for the species-specific nature of fungal recognition via DC-SIGN. The discovered highly specific oligosaccharide ligands of DC-SIGN are tractable tools for interdisciplinary investigations of mechanisms of fungal innate immunity and anti- Candida defense. Ligand- and receptor-based NMR data demonstrated the pentasaccharide-to-DC-SIGN interaction in solution and enabled the deciphering of the interaction topology.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2023 The Author(s).)
Databáze: MEDLINE
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