Antineoplastic effects of sodium dichloroacetate and omeprazole, alone or in combination, on canine oral mucosal melanoma cells.

Autor:	Toledo GF; Laboratory of Experimental and Comparative Oncology, School of Veterinary Medicine and Animal Science of the University of São Paulo, São Paulo, Brazil., Nagamine MK; Laboratory of Experimental and Comparative Oncology, School of Veterinary Medicine and Animal Science of the University of São Paulo, São Paulo, Brazil., Nowosh V; Laboratory of Comparative Imuno-Oncology, School of Veterinary Medicine and Animal Science of the University of São Paulo, São Paulo, Brazil., Machado FT; Laboratory of Mitochondrial Genetics, Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil., Massoco CO; Laboratory of Comparative Imuno-Oncology, School of Veterinary Medicine and Animal Science of the University of São Paulo, São Paulo, Brazil., Souza-Pinto NC; Laboratory of Mitochondrial Genetics, Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil., Dagli MLZ; Laboratory of Experimental and Comparative Oncology, School of Veterinary Medicine and Animal Science of the University of São Paulo, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2023 Jul 13; Vol. 10, pp. 1186650. Date of Electronic Publication: 2023 Jul 13 (Print Publication: 2023).
DOI:	10.3389/fvets.2023.1186650
Abstrakt:	Oral mucosal melanoma (OMM) is a common neoplasm in canines, although it is rare in humans. Cancer cells present alterations in energetic metabolism, and the Warburg effect states that most cancer cells undergo aerobic glycolysis. This can be reversed by certain drugs, resulting in decreased cell viability and cell death. We sought to evaluate the effects of sodium dichloroacetate (DCA) and omeprazole (OMP) alone or in combination on canine OMM and human melanoma cells. CMGD5 and SK-MEL-28 cell lines were treated with DCA and OMP alone or in combination, and cell viability was assessed using the crystal violet assay. Cell death (apoptosis and necrosis) was assessed by Annexin V and propidium iodide (PI) staining assays using flow cytometry. In addition, the oxygen consumption rate (OCR) was evaluated using a SeaHorse XF assay. Treatment with DCA or OMP alone resulted in a significant, but not dose-dependent, reduction in cell viability in both cell lines; however, the combination of DCA and OMP resulted in a significant and dose-dependent decrease in viability in both cell lines. DCA and OMP, alone or in combination, did not alter OCR at the concentrations tested in either cell line. Since the combination of DCA and OMP potentialized the inhibition of viability and increased cell death in a synergistic manner in melanoma cells, this approach may represent a new repurposing strategy to treat cancer. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Toledo, Nagamine, Nowosh, Machado, Massoco, Souza-Pinto and Dagli.)
Databáze:	MEDLINE
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