Investigation of SARS-CoV-2 presence on environmental surfaces and waste in healthcare and non-healthcare facilities.

Autor:	Mai LTP; Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam., Tran VD; Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam., Phuong HVM; Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam., Trang UTH; Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam., Thanh LT; Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam., Son NV; Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam., Cuong VD; Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam., Dung LP; Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam., Hanh NTM; Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam., Hai H; Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam., Oanh DTK; Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam., Thuy NT; Department of Public Health, National Institute of Hygiene and Epidemiology, 1 Yersin St., Hai Ba Trung Dist, Hanoi, Vietnam.
Jazyk:	angličtina
Zdroj:	Environmental challenges (Amsterdam, Netherlands) [Environ Chall (Amst)] 2022 Apr; Vol. 7, pp. 100526. Date of Electronic Publication: 2022 Apr 22.
DOI:	10.1016/j.envc.2022.100526
Abstrakt:	Objective: The objective of the paper is to investigate the presence of SARS-CoV-2 on inanimate surfaces in four healthcare facilities treating patients with COVID-19 and four quarantine regiments of provincial military commands. Methods: From August to October 2020, a total of 468 one-off environmental samples consisting of inanimate surfaces, garbage, and wastewater were collected. The real-time RT-PCR assay targeting E and RdRp genes to detect SARS-CoV-2 and checklist and questionnaire of disinfection practices were employed. If detected by RT-PCR, then positive samples are subjected to cell culture to determine viability. Results: The test results showed all samples (100%) to be negative with SARS-CoV-2 resulting in unperformed virus culture. As for recent disinfection practices, chlorine-based products dissolved at a concentration of 0.1% (1000 ppm) in the general context or 0.5% (5000 ppm) for blood and body fluid spills are routinely applied twice a day and at the discharge of patients or quarantined people. Conclusions: The finding may illustrate the importance of disinfection practices in removing pathogens or significantly reducing SARS-CoV-2 contamination on environmental surfaces and waste. Competing Interests: The authors declare that they have no conflict of interest. (© 2022 The Author(s).)
Databáze:	MEDLINE
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