A kinetic characterization of the gill V(H + )-ATPase from two hololimnetic populations of the Amazon River shrimp Macrobrachium amazonicum.

Autor: Fabri LM; Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Brazil., Garçon DP; Universidade Federal do Triângulo Mineiro, Campus Universitário de Iturama, 38280-000, Brazil., Moraes CM; Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Brazil., Pinto MR; Laboratory of Biopathology and Molecular Biology, University of Uberaba, Uberaba, Minas Gerais, Brazil., McNamara JC; Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14040-901, Brazil.; Centro de Biologia Marinha, Universidade de São Paulo, São Sebastião, 11000-600, Brazil. Electronic address: https://twitter.com/maracoani., Leone FA; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14040-901, Brazil. Electronic address: fdaleone@ffclrp.usp.br.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2023 Oct-Dec; Vol. 268, pp. 110880. Date of Electronic Publication: 2023 Jul 28.
DOI: 10.1016/j.cbpb.2023.110880
Abstrakt: This investigation examines the kinetic characteristics and effect of acclimation to a brackish medium (21 ‰S) on gill V(H + )-ATPase activity in two hololimnetic populations of M. amazonicum. We also investigate the cellular immunolocalization of the enzyme. Immunofluorescence findings demonstrate that the V(H + )-ATPase c-subunit is distributed in the apical pillar cells of shrimps in fresh water but is absent after acclimation to 21 ‰S for 10 days. V(H + )-ATPase activity from the Tietê River population is ≈50% greater than the Grande River population, comparable to a wild population from the Santa Elisa Reservoir, but is 2-fold less than in cultivated shrimps. V(H + )-ATPase activity in the Tietê and the Grande River shrimps is abolished after 21 ‰S acclimation. The apparent affinities of the V(H + )-ATPase for ATP (0.27 ± 0.04 and 0.16 ± 0.03 mmol L -1 , respectively) and Mg 2+ (0.28 ± 0.05 and 0.14 ± 0.02 mmol L -1 , respectively) are similar in both populations. The absence of V(H + )-ATPase activity in salinity-acclimated shrimps and its apical distribution in shrimps in fresh water underpins the importance of the crustacean V(H + )-ATPase for ion uptake in fresh water.
Competing Interests: Declaration of Competing Interest All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest or non-financial interest in the subject matter or materials discussed in this manuscript.
(Published by Elsevier Inc.)
Databáze: MEDLINE
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