| Autor: |
Loginova TP; A.N. Nesmeyanov Instituite of Organoelement Compounds of Russian Academy of Sciences, Vavilova Street 28, 119334 Moscow, Russia., Khotina IA; A.N. Nesmeyanov Instituite of Organoelement Compounds of Russian Academy of Sciences, Vavilova Street 28, 119334 Moscow, Russia., Kabachii YA; A.N. Nesmeyanov Instituite of Organoelement Compounds of Russian Academy of Sciences, Vavilova Street 28, 119334 Moscow, Russia., Kochev SY; A.N. Nesmeyanov Instituite of Organoelement Compounds of Russian Academy of Sciences, Vavilova Street 28, 119334 Moscow, Russia., Abramov VM; JSC Institute Immunological Engineering, Nauchnaya street 1, 142380 Lybuchany, Moscow District, Moscow Region, Russia., Khlebnikov VS; JSC Institute Immunological Engineering, Nauchnaya street 1, 142380 Lybuchany, Moscow District, Moscow Region, Russia., Kulikova NL; JSC Institute Immunological Engineering, Nauchnaya street 1, 142380 Lybuchany, Moscow District, Moscow Region, Russia., Mezhuev YO; A.N. Nesmeyanov Instituite of Organoelement Compounds of Russian Academy of Sciences, Vavilova Street 28, 119334 Moscow, Russia.; Department of Biomaterials, Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia. |
| Abstrakt: |
Cationic copolymers based on 2-(N, N -dimethylamino)ethyl methacrylate and polyethylene glycol monomethyl ether (pDMAEMA-co-PEO) with different molecular weights have been synthesized. Their physicochemical properties were studied by NMR spectroscopy, sedimentation, and potentiometric titration. According to the data of potentiometric titration for the synthesized pegylated cationic copolymers, the apparent dissociation constants were determined in the pH range from 4.5 to 8.5. The physicochemical properties of interpolyelectrolyte complexes of these polycations with circular DNA (IPEC DNA) were also studied by dynamic light scattering, electrophoretic mobility, and TEM methods. It has been established that the diameter and electrokinetic potential (ζ-potential) of interpolyelectrolyte complexes can be varied over a wide range (from 200 nm to 1.5 μm and from -25 mV to +30 mV) by changing the ratio of oppositely charged ionizable groups in pegylated cationic copolymers and DNA, as well as by regulating medium pH. The resistance of the IPEC DNA/polycation complex to the action of nucleases was studied by electrophoresis in agarose gel; the cytotoxic effect of the polymers in vitro, and the efficiency of penetration (transfection) of IPEC DNA with PDMAEMA-co-PEO-polycations into eukaryotic cells of a cell line derived from human embryonic kidneys HEK 293 in vitro. |
