| Autor: |
Elferjane MR; Faculty of Nursing and Health Sciences, University of Misurata, Alshowahda Park, 3rd Ring Road, Misurata 2478, Libya.; Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia., Jovanović AA; Institute for the Application of the Nuclear Energy INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia., Milutinović V; Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia., Čutović N; Institute for Medicinal Plant Research 'Dr Josif Pančić', Tadeuša Košćuška 1, 11000 Belgrade, Serbia., Jovanović Krivokuća M; Institute for the Application of the Nuclear Energy INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia., Marinković A; Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia. |
| Abstrakt: |
In the study, the optimization of the extraction from Aloe vera leaf waste was performed via varying solid-to-solvent ratio, solvent type, extraction time, and technique (maceration, heat-, ultrasound-, and microwave-assisted extractions-HAE, UAE, and MAE, respectively). The optimal extraction conditions for achieving the highest polyphenol content are a 1:30 ratio, 70% ethanol, and 30 min of HAE. Total flavonoid and protein contents were significantly higher in the extract from MAE, while total condensed tannin content was the highest in HAE. LC-MS analysis quantified 13 anthraquinone and chromone compounds. The variations in the FT-IR spectra of the extracts obtained by different extraction procedures are minor. The influence of extraction conditions on the antioxidant ability of the extracts depended on applied antioxidant assays. The extracts possessed medium inhibition properties against Staphylococcus aureus and weak inhibitory activity against Enterococcus feacalis . The extracts had stimulative effect on HaCaT cell viability. Regarding the extraction yield, there was a significant difference between the used extraction techniques (MAE > HAE > maceration and UAE). The presented study is an initial step in the production of polyphenol-rich extracts from A. vera leaf waste aimed to be used for the potential preparation of pharmaceutical and cosmetic formulations for the skin. |
