| Autor: |
Maiztegui B; CENEXA, Centro de Endocrinología Experimental y Aplicada (UNLP-CONICET La Plata), La Plata 1900, Argentina.; Facultad de Ciencias Médicas, Universidad Nacional de la Plata, La Plata 1900, Argentina., Villagarcía HG; CENEXA, Centro de Endocrinología Experimental y Aplicada (UNLP-CONICET La Plata), La Plata 1900, Argentina.; Facultad de Ciencias Médicas, Universidad Nacional de la Plata, La Plata 1900, Argentina., Román CL; CENEXA, Centro de Endocrinología Experimental y Aplicada (UNLP-CONICET La Plata), La Plata 1900, Argentina.; Facultad de Ciencias Médicas, Universidad Nacional de la Plata, La Plata 1900, Argentina., Flores LE; CENEXA, Centro de Endocrinología Experimental y Aplicada (UNLP-CONICET La Plata), La Plata 1900, Argentina.; Facultad de Ciencias Médicas, Universidad Nacional de la Plata, La Plata 1900, Argentina., Prieto JM; Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, UK., Castro MC; CENEXA, Centro de Endocrinología Experimental y Aplicada (UNLP-CONICET La Plata), La Plata 1900, Argentina.; Facultad de Ciencias Médicas, Universidad Nacional de la Plata, La Plata 1900, Argentina., Massa ML; CENEXA, Centro de Endocrinología Experimental y Aplicada (UNLP-CONICET La Plata), La Plata 1900, Argentina., Schinella GR; Facultad de Ciencias Médicas, Universidad Nacional de la Plata, La Plata 1900, Argentina.; Instituto de Ciencias de la Salud, UNAJ-CICPBA, Florencio Varela 1888, Argentina., Francini F; CENEXA, Centro de Endocrinología Experimental y Aplicada (UNLP-CONICET La Plata), La Plata 1900, Argentina.; Facultad de Ciencias Médicas, Universidad Nacional de la Plata, La Plata 1900, Argentina. |
| Abstrakt: |
"Yerba mate" (YM), an aqueous extract of Ilex paraguariensis, has antioxidant, diuretic, cardio-protective and hypoglycaemic properties. Since its effect on the pancreatic islets remains unclear, we evaluated insulin sensitivity and glucose-stimulated insulin secretion (GSIS) in rats consuming YM or tap water (C) for 21 days. Glucose tolerance, glycemia, triglyceridemia, insulinemia, TBARS and FRAP serum levels were evaluated. GSIS and mRNA levels of insulin signaling pathway and inflammatory markers were measured in isolated pancreatic islets from both groups. In C rats, islets were incubated with YM extract or its phenolic components to measure GSIS. YM improved glucose tolerance, enhanced GSIS, increased FRAP plasma levels and islet mRNA levels of IRS-1 and PI3K (p110), and decreased TBARS plasma levels and islet gene expression of TNF-α and PAI-1. Islets from C rats incubated with 100 µg/mL dry YM extract, 1 µM chlorogenic acid, 0.1 and 1 µM rutin, 1 µM caffeic acid or 1 µM quercetin showed an increase in GSIS. Our results suggest that YM enhances glucose tolerance because of its positive effects on GSIS, oxidative stress rate and insulin sensitivity in rat islets, suggesting that long-term dietary supplementation with YM may improve glucose homeostasis in pre-diabetes or type 2 diabetes. |
