Hetero-Pathogenic O181:H4 EAHEC Strain of Sequence Type ST678 Associated with Hemolytic-Uremic Syndrome in Schoolchildren in Russia.

Autor:	Kartsev NN; Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Detusheva EV; Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Kalmantaeva OV; Laboratory of Molecular Biology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Korobova OV; Laboratory of Biological Trials, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Gerasimov VN; Department of Disinfectology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Kombarova TI; Laboratory of Biological Trials, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Borzilov AI; Laboratory of Biological Trials, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Fursova NK; Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Vereshchagin AN; N. D. Zelinsky Institute of Organic Chemistry, Leninsky pr. 47, 119991 Moscow, Russia., Svetoch EA; Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia.
Jazyk:	angličtina
Zdroj:	Microorganisms [Microorganisms] 2023 Jul 07; Vol. 11 (7). Date of Electronic Publication: 2023 Jul 07.
DOI:	10.3390/microorganisms11071771
Abstrakt:	Background: In the last decade, the importance of hetero-pathogenic enteroaggregative Shiga-toxin-producing E. coli for public health has increased. Recently, we described the genetic background of the EAHEC O181:H4 strain of ST678 carrying the stx2 gene in prophage and five plasmids, including the plasmid-carrying aggR and aaiC genes. Here, we present the morphological and enzymatic characteristics of this strain, as well as susceptibility to antimicrobials, biofilm formation, etc. Methods: Bacterial morphology was studied using an electron microscope. Susceptibility to antimicrobials was determined using the microdilution method. Cytotoxicity was estimated in Vero cells. Virulence was studied on mice. Results: The morphological and enzymatic properties of the hetero-pathogenic EAHEC strain were typical for E. coli ; electron microscopy revealed the specific flagella. The strain was susceptible to most antibiotics and disinfectants but resistant to ampicillin and ciprofloxacin and showed a high degree of biofilm formation. Cytotoxicity towards Vero cells was estimated as 80%. Conclusions: The emergence of a new O181:H4 EAHEC strain poses a potential threat to humans because of the virulence potential that must be taken into account in the epidemiological analysis of outbreaks and sporadic cases of foodborne infections associated with hemolytic-uremic syndrome.
Databáze:	MEDLINE
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