Listeria monocytogenes Strains Persisting in a Meat Processing Plant in Central Italy: Use of Whole Genome Sequencing and In Vitro Adhesion and Invasion Assays to Decipher Their Virulence Potential.

Autor:	Schiavano GF; Dipartimento di Studi Umanistici, Università degli Studi di Urbino 'Carlo Bo', Via Bramante, 17, 61029 Urbino, Italy., Guidi F; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Laboratorio Nazionale di Riferimento per Listeria Monocytogenes, Via Campo Boario, 64100 Teramo, Italy., Pomilio F; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Laboratorio Nazionale di Riferimento per Listeria Monocytogenes, Via Campo Boario, 64100 Teramo, Italy., Brandi G; Dipartimento di Scienze Biomolecolari, Università degli Studi di Urbino 'Carlo Bo', Via Santa Chiara, 27, 61029 Urbino, Italy., Salini R; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Centro Operativo Veterinario per l'Epidemiologia, Programmazione, Informazione e Analisi del Rischio (COVEPI), National Reference Center for Veterinary Epidemiology, Via Campo Boario, 64100 Teramo, Italy., Amagliani G; Dipartimento di Scienze Biomolecolari, Università degli Studi di Urbino 'Carlo Bo', Via Santa Chiara, 27, 61029 Urbino, Italy., Centorotola G; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Laboratorio Nazionale di Riferimento per Listeria Monocytogenes, Via Campo Boario, 64100 Teramo, Italy., Palma F; Dipartimento di Scienze Biomolecolari, Università degli Studi di Urbino 'Carlo Bo', Via Santa Chiara, 27, 61029 Urbino, Italy., Felici M; Dipartimento di Scienze Biomolecolari, Università degli Studi di Urbino 'Carlo Bo', Via Santa Chiara, 27, 61029 Urbino, Italy., Lorenzetti C; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche 'Togo Rosati', Via Gaetano Salvemini, 1, 06126 Perugia, Italy., Blasi G; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche 'Togo Rosati', Via Gaetano Salvemini, 1, 06126 Perugia, Italy.
Jazyk:	angličtina
Zdroj:	Microorganisms [Microorganisms] 2023 Jun 26; Vol. 11 (7). Date of Electronic Publication: 2023 Jun 26.
DOI:	10.3390/microorganisms11071659
Abstrakt:	In this study, we used both a WGS and an in vitro approach to study the virulence potential of nine Listeria monocytogenes ( Lm ) strains belonging to genetic clusters persisting in a meat processing plant in Central Italy. The studied clusters belonged to CC1-ST1, CC9-ST9, and CC218-ST2801. All the CC1 and CC218 strains presented the same accessory virulence genes (LIPI-3, gltA, gltB, and aut_IVb). CC1 and CC9 strains presented a gene profile similarity of 22.6% as well as CC9 and CC218 isolates. CC1 and CC218 showed a similarity of 45.2% of the same virulence profile. The hypervirulent strains of lineage I (CC1 and CC218) presented a greater ability to adhere and invade Caco-2 cells than hypovirulent ones (CC9). CC1 strains were significantly more adhesive and invasive compared with CC9 and CC218 strains, although these last CCs presented the same accessory virulence genes. No statistically significant difference was found comparing CC218 with CC9 strains. This study provided for the first time data on the in vitro adhesiveness and invasiveness of CC218-ST2801 and added more data on the virulence characteristics of CC1 and CC9. What we observed confirmed that the ability of Lm to adhere to and invade human cells in vitro is not always decipherable from its virulence gene profile.
Databáze:	MEDLINE
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