Development and Validation of Sensitive, Fast and Simple LC-MS/MS Method to Investigate the Association between Adrenocortical Steroidogenesis and the High Intensity Exercise in Elite Athletes.
| Autor: | Dikunets MA; Federal Science Center of Physical Culture and Sport, Elizavetinsky Lane 10/1, 105005 Moscow, Russia., Dudko GA; Federal Science Center of Physical Culture and Sport, Elizavetinsky Lane 10/1, 105005 Moscow, Russia., Virus ED; Federal Science Center of Physical Culture and Sport, Elizavetinsky Lane 10/1, 105005 Moscow, Russia.; Institute of General Pathology and Pathophysiology, Baltiyskaya St. 8, 125315 Moscow, Russia. |
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| Jazyk: | angličtina |
| Zdroj: | Metabolites [Metabolites] 2023 Jul 05; Vol. 13 (7). Date of Electronic Publication: 2023 Jul 05. |
| DOI: | 10.3390/metabo13070825 |
| Abstrakt: | The very high intensity of exercise accompanied by mental stress triggers adaptive mechanisms associated with adrenocortical steroidogenesis. However, the association between adrenocortical steroidogenesis and the high intensity of exercise in elite athletes is poorly studied. A significant obstacle to solving this complex task is the wide range (4-5 orders) of steroid concentrations in serum and limitations related to the amount of biological samples taken from professional athletes. To solve this task, we have developed and validated a non-trivial approach for targeted serum metabolic profiling based on the use of LC-MS/MS with dual-polarity electrospray ionization. The developed method based on the proposed approach allows for the quantitative determination of 14 stress resistance biomarkers in elite athletes using a small amount of specimen within 8.5 min. |
| Databáze: | MEDLINE |
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