| Autor: |
Ichihashi M; Kobe University, Kobe 657-8501, Japan.; Arts Ginza Clinic, Tokyo 105-0004, Japan., Tanaka M; Arts Ginza Clinic, Tokyo 105-0004, Japan., Iizuka T; Arts Ginza Clinic, Tokyo 105-0004, Japan., Totsuka H; Arts Ginza Clinic, Tokyo 105-0004, Japan., Tominaga E; Arts Ginza Clinic, Tokyo 105-0004, Japan., Hitomi Y; Arts Ginza Clinic, Tokyo 105-0004, Japan., Ando H; Department of Applied Chemistry and Biotechnology, Okayama University of Science, Okayama 700-0005, Japan., Nishikata T; Frontier of Innovative Research in Science and Technology, Konan University, Kobe 658-8501, Japan., Mizutani KI; Laboratory of Stem Cell Biology, Graduate School of Pharmaceutical Science, Kobe Gakuin University, Kobe 650-8586, Japan. |
| Abstrakt: |
Facial skin aging is the most visible manifestation of aging in the body. In this study, we aimed to rejuvenate aging skin via a one-time intradermal injection of autologous adipose-derived stem cells (ADSCs). Eight patients were enrolled for study. Photographs of patients taken immediately before and 1, 3, 6, and 12 months after ADSC injections were comparatively evaluated for visible skin manifestations. ADSCs were cultured from the abdominal-skin-derived subcutaneous fat tissue, and 1 × 108 cultured ADSCs were injected intradermally into the facial skin. Cultured myoblasts were incubated with the supernatant derived from ADSCs, and the effect was evaluated via glucose consumption and lactic acid production in the medium. Eight cases showed the shallowing and disappearance of wrinkles, including those of the glabella, lower eyelids, crow`s feet, and forehead and nasolabial grooves, a month to several months after treatment. Double eyelids became prominent, and facial pores significantly reduced in size. These effects lasted for over one year. Myoblasts cultured in the presence of an ADSC-derived exosome were activated compared to that of ADSCs cultured without supernatant. The result supports the role of muscle in ADSC skin rejuvenation. The present study first reports that a single intradermal administration of cultured ADSCs rejuvenates aged facial skin over the course of one year. Further, patients exhibited definite double eyelids and pore shrinkage, strongly indicating the active involvement of muscle, which was supported by an in vitro study. Our study also suggested the important role of biological factors delivered from injected stem cells, although the detailed mechanism of rejuvenation effects of ADSC skin injection remains to be clarified. |
