| Autor: |
Oprică GM; National Institute for Research and Development in Chemistry and Petrochemistry, 202 Spl. Independentei, 060021 Bucharest, Romania., Panaitescu DM; National Institute for Research and Development in Chemistry and Petrochemistry, 202 Spl. Independentei, 060021 Bucharest, Romania., Usurelu CD; National Institute for Research and Development in Chemistry and Petrochemistry, 202 Spl. Independentei, 060021 Bucharest, Romania.; Faculty of Chemical Engineering and Biotechnology, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania., Vlăsceanu GM; Faculty of Chemical Engineering and Biotechnology, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania., Stanescu PO; Faculty of Chemical Engineering and Biotechnology, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania., Lixandru BE; Cantacuzino National Medical-Military Institute for Research and Development, 103 Spl. Independentei, 050096 Bucharest, Romania., Vasile V; Cantacuzino National Medical-Military Institute for Research and Development, 103 Spl. Independentei, 050096 Bucharest, Romania., Gabor AR; National Institute for Research and Development in Chemistry and Petrochemistry, 202 Spl. Independentei, 060021 Bucharest, Romania., Nicolae CA; National Institute for Research and Development in Chemistry and Petrochemistry, 202 Spl. Independentei, 060021 Bucharest, Romania., Ghiurea M; National Institute for Research and Development in Chemistry and Petrochemistry, 202 Spl. Independentei, 060021 Bucharest, Romania., Frone AN; National Institute for Research and Development in Chemistry and Petrochemistry, 202 Spl. Independentei, 060021 Bucharest, Romania. |
| Abstrakt: |
Nanocellulose (NC) is a valuable material in tissue engineering, wound dressing, and drug delivery, but its lack of antimicrobial activity is a major drawback for these applications. In this work, basil ethanolic extract (BE) and basil seed mucilage (BSM) were used to endow nanocellulose with antibacterial activity. NC/BE and NC/BE/BSM sponges were obtained from nanocellulose suspensions and different amounts of BE and BSM after freeze-drying. Regardless of the BE or BSM content, the sponges started to decompose at a lower temperature due to the presence of highly volatile active compounds in BE. A SEM investigation revealed an opened-cell structure and nanofibrillar morphology for all the sponges, while highly impregnated nanofibers were observed by SEM in NC/BE sponges with higher amounts of BE. A quantitative evaluation of the porous morphology by microcomputer tomography showed that the open porosity of the sponges varied between 70% and 82%, being lower in the sponges with higher BE/BSM content due to the impregnation of cellulose nanofibers with BE/BSM, which led to smaller pores. The addition of BE increased the specific compression strength of the NC/BE sponges, with a higher amount of BE having a stronger effect. A slight inhibition of S. aureus growth was observed in the NC/BE sponges with a higher amount of BE, and no effect was observed in the unmodified NC. In addition, the NC/BE sponge with the highest amount of BE and the best antibacterial effect in the series showed no cytotoxic effect and did not interfere with the normal development of the L929 cell line, similar to the unmodified NC. This work uses a simple, straightforward method to obtain highly porous nanocellulose structures containing antibacterial basil extract for use in biomedical applications. |
