Gold Nanoparticles for Monitoring of Mesenchymal Stem-Cell-Loaded Bioresorbable Polymeric Wraps for Arteriovenous Fistula Maturation.

Autor: Barcena AJR; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.; College of Medicine, University of the Philippines Manila, Manila 1000, Philippines., Perez JVD; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.; College of Medicine, University of the Philippines Manila, Manila 1000, Philippines., Damasco JA; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Bernardino MR; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., San Valentin EMD; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Klusman C; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.; School of Medicine, Baylor College of Medicine, Houston, TX 77030, USA., Martin B; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.; School of Medicine, Baylor College of Medicine, Houston, TX 77030, USA., Cortes A; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Canlas GM; Department of Chemistry, Lamar University, Beaumont, TX 77710, USA., Del Mundo HC; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Heralde FM 3rd; College of Medicine, University of the Philippines Manila, Manila 1000, Philippines., Avritscher R; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Fowlkes N; Department of Veterinary Medicine and Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Bouchard RR; Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Cheng J; Section of Nephrology, Department of Medicine, Selzman Institute for Kidney Health, Baylor College of Medicine, Houston, TX 77030, USA., Huang SY; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Melancon MP; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.; The University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences, Houston, TX 77030, USA.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 Jul 21; Vol. 24 (14). Date of Electronic Publication: 2023 Jul 21.
DOI: 10.3390/ijms241411754
Abstrakt: Mesenchymal stem cell (MSC)-seeded polymeric perivascular wraps have been shown to enhance arteriovenous fistula (AVF) maturation. However, the wraps' radiolucency makes their placement and integrity difficult to monitor. Through electrospinning, we infused gold nanoparticles (AuNPs) into polycaprolactone (PCL) wraps to improve their radiopacity and tested whether infusion affects the previously reported beneficial effects of the wraps on the AVF's outflow vein. Sprague Dawley rat MSCs were seeded on the surface of the wraps. We then compared the effects of five AVF treatments-no perivascular wrap (i.e., control), PCL wrap, PCL + MSC wrap, PCL-Au wrap, and PCL-Au + MSC wrap-on AVF maturation in a Sprague Dawley rat model of chronic kidney disease (n = 3 per group). Via micro-CT, AuNP-infused wraps demonstrated a significantly higher radiopacity compared to that of the wraps without AuNPs. Wraps with and without AuNPs equally reduced vascular stenoses, as seen via ultrasonography and histomorphometry. In the immunofluorescence analysis, representative MSC-seeded wraps demonstrated reduced neointimal staining for markers of infiltration with smooth muscle cells (α-SMA), inflammatory cells (CD45), and fibroblasts (vimentin) compared to that of the control and wraps without MSCs. In conclusion, AuNP infusion allows in vivo monitoring via micro-CT of MSC-seeded polymeric wraps over time, without compromising the benefits of the wrap for AVF maturation.
Databáze: MEDLINE
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