Single-cell profiling of prurigo nodularis demonstrates immune-stromal crosstalk driving profibrotic responses and reversal with nemolizumab.

Autor: Ma F; Department of Dermatology, University of Michigan, Ann Arbor, Mich., Gharaee-Kermani M; Department of Dermatology, University of Michigan, Ann Arbor, Mich., Tsoi LC; Department of Dermatology, University of Michigan, Ann Arbor, Mich; Department of Biostatistics, University of Michigan, Ann Arbor, Mich; Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, Mich., Plazyo O; Department of Dermatology, University of Michigan, Ann Arbor, Mich., Chaskar P; Galderma, Lausanne, Switzerland., Harms P; Department of Dermatology, University of Michigan, Ann Arbor, Mich; Department of Pathology, University of Michigan, Ann Arbor, Mich., Patrick MT; Department of Dermatology, University of Michigan, Ann Arbor, Mich., Xing X; Department of Dermatology, University of Michigan, Ann Arbor, Mich., Hile G; Department of Dermatology, University of Michigan, Ann Arbor, Mich., Piketty C; Galderma, Lausanne, Switzerland., Lazzari A; Galderma, Lausanne, Switzerland., Van Delm W; Galderma, Lausanne, Switzerland., Maverakis E; Department of Dermatology, University of California-Davis, Sacramento, Calif., Nakamura M; Department of Dermatology, University of Michigan, Ann Arbor, Mich., Modlin RL; Department of Dermatology, University of California-Los Angeles, Calif., Kahlenberg JM; Department of Internal Medicine, Division of Rheumatology, University of Michigan, Ann Arbor, Mich; Taubman Medical Research Institute, University of Michigan, Ann Arbor, Mich., Billi AC; Department of Dermatology, University of Michigan, Ann Arbor, Mich., Julia V; Galderma, Lausanne, Switzerland., Krishnaswamy JK; Galderma, Lausanne, Switzerland., Gudjonsson JE; Department of Dermatology, University of Michigan, Ann Arbor, Mich; Department of Internal Medicine, Division of Rheumatology, University of Michigan, Ann Arbor, Mich; Taubman Medical Research Institute, University of Michigan, Ann Arbor, Mich. Electronic address: johanng@med.umich.edu.
Jazyk: angličtina
Zdroj: The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 2024 Jan; Vol. 153 (1), pp. 146-160. Date of Electronic Publication: 2023 Jul 26.
DOI: 10.1016/j.jaci.2023.07.005
Abstrakt: Background: Prurigo nodularis (PN) is a chronic neuroimmune skin disease characterized by bilaterally distributed pruritic hyperkeratotic nodules on extremities and trunk. Neuroimmune dysregulation and chronic scratching are believed to both induce and maintain the characteristic lesions.
Objectives: This study sought to provide a comprehensive view of the molecular pathogenesis of PN at the single-cell level to identify and outline key pathologic processes and the cell types involved. Features that distinguish PN skin from the skin of patients with atopic dermatitis were of particular interest. We further aimed to determine the impact of the IL31RA antagonist, nemolizumab, and its specificity at the single-cell level.
Methods: Single-cell RNA-sequencing of skin from 15 healthy donors and nonlesional and lesional skin from 6 patients each with PN and atopic dermatitis, combined with spatial-sequencing using the 10x Visium platform. Integration with bulk RNA-sequencing data from patients treated with nemolizumab.
Results: This study demonstrates that PN is an inflammatory skin disease characterized by both keratinocyte proliferation and activation of profibrotic responses. This study also demonstrates that the COL11A1 + fibroblast subset is a major contributor to fibrosis and is predominantly found in the papillary dermis of PN skin. Activation of fibrotic responses is the main distinguishing feature between PN and atopic dermatitis skin. This study further shows the broad effect of nemolizumab on PN cell types, with a prominent effect driving COL11A1 + fibroblast and keratinocyte responses toward normal.
Conclusions: This study provides a high-resolution characterization of the cell types and cellular processes activated in PN skin, establishing PN as a chronic fibrotic inflammatory skin disease. It further demonstrates the broad effect of nemolizumab on pathological processes in PN skin.
(Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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