The protective effects of hyperoxic pre-treatment in human-derived adipose tissue mesenchymal stem cells against in vitro oxidative stress and a rat model of renal ischaemia-reperfusion.

Autor:	Ahmadi Somaghian S; Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran., Pajouhi N; Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran., Dezfoulian O; Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran., Pirnia A; Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran., Kaeidi A; Physiology-Pharmacology Research Center, Research Institute of Basic Medical Science, Rafsanjan University of Medical Sciences, Rafsanjan, Iran., Rasoulian B; Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Jazyk:	angličtina
Zdroj:	Archives of physiology and biochemistry [Arch Physiol Biochem] 2023 Jul 28, pp. 1-10. Date of Electronic Publication: 2023 Jul 28.
DOI:	10.1080/13813455.2023.2238918
Abstrakt:	Objective: Improvement of cell survival is essential for achieving better clinical outcomes in stem cell therapy. We investigated the effects of hyperoxic pre-treatment (HP) on the viability of human adipose stromal stem cells (ASCs). Materials and Methods: MTT and Western blot tests were used to assess cell viability and the expression of apoptosis-related proteins, respectively. For the in-vivo trial, the rats were subjected to renal ischaemia-reperfusion (IR). Results: The results showed that HP could significantly increase the viability of ASCs and decrease apoptotic markers (Bax/BCL-2 ratio and Caspase-3) compared with control cells. There were some additional effects with regard to the improvement of renal structure and function in the animal model. However, the difference between the treated and non-treated transplanted ASCs failed to reach significance. Conclusion: These results suggested that HP could increase the survival of ASCs against oxidative stress-induced damages in the in-vitro condition, but this strategy was not highly effective in renal IR.
Databáze:	MEDLINE
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