Virulence of Shigatoxigenic and Enteropathogenic Escherichia coli O80:H2 in Galleria mellonella Larvae: Comparison of the Roles of the pS88 Plasmids and STX2d Phage.

Autor: Ikeda R; Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, Belgium., Laforêt F; Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, Belgium., Antoine C; Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, Belgium., Adachi M; Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, Belgium., Nakamura K; Department of Bacteriology, Faculty of Medical Science, Kyushu University, Fukuoka 812-8582, Japan., Habets A; Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, Belgium., Kler C; Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, Belgium., De Rauw K; Belgium National Reference Center of STEC (NRC STEC), Universitair Ziekenhuis Brussel (UZ Brussel), Vrije Universiteit Brussel (VUB), B-1090 Brussels, Belgium., Hayashi T; Department of Bacteriology, Faculty of Medical Science, Kyushu University, Fukuoka 812-8582, Japan., Mainil JG; Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, Belgium., Thiry D; Veterinary Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Center for Fundamental and Applied Research for Animals and Health (FARAH), University of Liège, B-4000 Liege, Belgium.
Jazyk: angličtina
Zdroj: Veterinary sciences [Vet Sci] 2023 Jun 29; Vol. 10 (7). Date of Electronic Publication: 2023 Jun 29.
DOI: 10.3390/vetsci10070420
Abstrakt: The invasiveness properties of Shigatoxigenic and enteropathogenic Escherichia coli (STEC and EPEC) O80:H2 in humans and calves are encoded by genes located on a pS88-like ColV conjugative plasmid. The main objectives of this study in larvae of the Galleria mellonella moth were therefore to compare the virulence of eight bovine STEC and EPEC O80:H2, of two E. coli pS88 plasmid transconjugant and STX2d phage transductant K12 DH10B, of four E. coli O80:non-H2, and of the laboratory E. coli K12 DH10B strains. Thirty larvae per strain were inoculated in the last proleg with 10 μL of tenfold dilutions of each bacterial culture corresponding to 10 to 10 6 colony-forming units (CFUs). The larvae were kept at 37 °C and their mortality rate was followed daily for four days. The main results were that: (i) not only the STEC and EPEC O80:H2, but also different E. coli O80:non-H2 were lethal for the larvae at high concentrations (from 10 4 to 10 6 CFU) with some variation according to the strain; (ii) the Stx2d toxin and partially the pS88 plasmid were responsible for the lethality caused by the E. coli O80:H2; (iii) the virulence factors of E. coli O80:non-H2 were not identified. The general conclusions are that, although the Galleria mellonella larvae represent a useful first-line model to study the virulence of bacterial pathogens, they are more limited in identifying their actual virulence properties.
Databáze: MEDLINE
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