| Autor: |
Louise V; Health and Nutrition Post-Graduate Program, Federal University of Ouro Preto, Ouro Preto 35402-145, Minas Gerais, Brazil., Machado BAA; Medical School, Federal University of Ouro Preto, Ouro Preto 35402-145, Minas Gerais, Brazil., Pontes WM; Health and Nutrition Post-Graduate Program, Federal University of Ouro Preto, Ouro Preto 35402-145, Minas Gerais, Brazil., Menezes TP; Health and Nutrition Post-Graduate Program, Federal University of Ouro Preto, Ouro Preto 35402-145, Minas Gerais, Brazil., Dias FCR; Department of Veterinary Medicine, Rural Federal University of Pernambuco, Recife 52171-900, Pernambuco, Brazil., Ervilhas LOG; Department of General Biology, Federal University of Viçosa, Viçosa 36570-000, Minas Gerais, Brazil., Pinto KMC; School of Physical Education, Federal University of Ouro Preto, Ouro Preto 35402-145, Mina Gerais, Brazil., Talvani A; Health and Nutrition Post-Graduate Program, Federal University of Ouro Preto, Ouro Preto 35402-145, Minas Gerais, Brazil.; Infectology and Tropical Medicine Post-Graduate Program, Federal University of Minas Gerais, Belo Horizonte 30130-100, Minas Gerais, Brazil. |
| Abstrakt: |
Theracurmin is a nanoparticle formulation derived from curcumin, a bioactive compound known for its antioxidant and anti-inflammatory properties. Trypanosoma cruzi , the etiological agent of Chagas disease, triggers an intense inflammatory response in mammals and also causes severe tissue damage. To evaluate the immunomodulatory and antiparasitic effects of Theracurmin, Swiss mice were experimentally infected with 10 3 trypomastigote forms of the Colombian strain of T. cruzi and submitted to daily therapy with 30 mg/kg of Theracurmin. In addition, daily benznidazole therapy (100 mg/kg) was performed as a positive control. We evaluated the systemic and tissue parasitism, the survival and the body mass rate, the release of inflammatory mediators (TNF, IL-6, IL-15, CCL2 and creatine kinase) and the tissue inflammation at day 30 post-infection. Theracurmin therapy reduced the parasitemia curve without altering the animals' survival rate, and it protected mice from losing body mass. Theracurmin also reduced CCL2 in cardiac tissue, IL-15 in cardiac and skeletal tissue, and plasma CK. Even without effects on TNF and IL-6 production and tissue amastigote nests, Theracurmin reduced the leukocyte infiltrate in both evaluated tissues, even in the case of more effective results observed to the benznidazole treatment. Our data suggest Theracurmin has an immunomodulatory (CCL2, IL-15, CK and tissue leukocyte infiltration) and a trypanocidal effect (on circulating parasites) during experimental infection triggered by the Colombian strain of T. cruzi . Further investigations are necessary to comprehend the Theracurmin role performed in combination with benznidazole or other potential anti- T. cruzi chemical compounds. |
