| Autor: |
Tofan L; Department of Natural Sciences, Faculty of Natural and Agricultural Sciences, Ovidius University of Constanța, 1 University Street, 900470 Constanța, Romania., Niță V; Marine Living Resources Department, National Institute for Marine Research and Development 'Grigore Antipa', 300 Mamaia Blvd., 900581 Constanța, Romania., Nenciu M; Marine Living Resources Department, National Institute for Marine Research and Development 'Grigore Antipa', 300 Mamaia Blvd., 900581 Constanța, Romania., Coatu V; Chemical Oceanography and Marine Pollution Department, National Institute for Marine Research and Development 'Grigore Antipa', 300 Mamaia Blvd., 900581 Constanța, Romania., Lazăr L; Chemical Oceanography and Marine Pollution Department, National Institute for Marine Research and Development 'Grigore Antipa', 300 Mamaia Blvd., 900581 Constanța, Romania., Damir N; Chemical Oceanography and Marine Pollution Department, National Institute for Marine Research and Development 'Grigore Antipa', 300 Mamaia Blvd., 900581 Constanța, Romania., Vasile D; Department of Natural Sciences, Faculty of Natural and Agricultural Sciences, Ovidius University of Constanța, 1 University Street, 900470 Constanța, Romania., Popoviciu DR; Department of Natural Sciences, Faculty of Natural and Agricultural Sciences, Ovidius University of Constanța, 1 University Street, 900470 Constanța, Romania., Brotea AG; Department of Natural Sciences, Faculty of Natural and Agricultural Sciences, Ovidius University of Constanța, 1 University Street, 900470 Constanța, Romania., Curtean-Bănăduc AM; Faculty of Sciences, The 'Lucian Blaga' University of Sibiu, 31 Victoriei Blvd., 550024 Sibiu, Romania., Avramescu S; Department of Inorganic Chemistry, Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 90 Șoseaua Panduri, 050663 Bucharest, Romania.; PROTMED Research Centre, University of Bucharest, 91-95 Splaiul Independenței, 050095 Bucharest, Romania., Aonofriesei F; Department of Natural Sciences, Faculty of Natural and Agricultural Sciences, Ovidius University of Constanța, 1 University Street, 900470 Constanța, Romania. |
| Abstrakt: |
The widespread use of Tebuconazole-based fungicides in phytosanitary treatments on a wide range of crops, on the one hand, and the lack of official reports on the amount of fungicide residues in nearby water basins, on the other hand, may lead to uncontrolled and hazardous contamination of water sources used by the resident population, and to serious effects on the environment and public health. Our study explores the acute toxicological risk of this fungicide on various organisms, from bacteria and yeast to fish, using a battery of tests (standardized Toxkit microbiotests and acute semi-static tests). By investigating the interaction between Tebuconazole and bacteria and yeast organisms, we observed that Gram-negative bacteria displayed a strong tolerance for Tebuconazole, while Gram-positive bacteria and yeasts proved to be very sensitive. The fish experiment was conducted on Chelon auratus juveniles exposed to five concentrations of the fungicide Tebustar EW (Tebuconazole, 250 g/L as active substance). After 96 h of exposure, the LC 50 for C. auratus was 1.13 mg/L. In the case of the Toxkit microbiotests' application, the following results were recorded: Spirodela polyrhiza EC 50 = 2.204 mg/L (after 72 h exposure), Thamnocephalus platyurus EC 50 = 0.115 mg/L (after 24 h), and Daphnia magna EC 50 = 2.37 mg/L (after 24-48 h). With the exception of bacteria and yeast, the same response pattern was observed for all non-target species tested; the response range expressed by concentrations causing growth inhibition or mortality was small, ranging between very close values that are quite low, thereby demonstrating the high toxicity of Tebuconazole-based fungicides to the environment. |
