| Autor: |
Mikheev VS; Department of Biochemistry and Biotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Gagarin Avenue 23, 603950 Nizhny Novgorod, Russia., Struchkova IV; Department of Biochemistry and Biotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Gagarin Avenue 23, 603950 Nizhny Novgorod, Russia.; LLC 'Mikofit', Internatsionalnaya 56b, 606440 Bor, Russia., Churkina LM; Department of Biochemistry and Biotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Gagarin Avenue 23, 603950 Nizhny Novgorod, Russia., Brilkina AA; Department of Biochemistry and Biotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Gagarin Avenue 23, 603950 Nizhny Novgorod, Russia., Berezina EV; Department of Biochemistry and Biotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Gagarin Avenue 23, 603950 Nizhny Novgorod, Russia. |
| Abstrakt: |
Oidiodendron maius G.L. Barron is a recognized fungal species capable of forming ericoid mycorrhiza with various positive effects on host plants; therefore, newly found and previously uncharacterized O. maius strains may be valuable for heather plants' controlled mycorrhization. Characteristics of the O. maius F3860 strain were studied, i.e., mycelium growth on various nutrient media and the ability to secrete auxins and enzymes. O. maius F3860 grew rapidly on malt extract agar and potato dextrose agar. It was also able to grow on nutrient media suitable for heather plant cultivation. The presence of the flavonoids rutin and quercetin increased the mycelium growth rate compared to the control, starting from the 8th to the 13th days of cultivation. The ability to secrete auxins was confirmed with bioassay and thin-layer chromatography, and their content, as well as phytase activity, was estimated spectrophotometrically. Both in nutrient media with tryptophan and without it, O. maius F3860 secreted about 6 μg IAA/mL growth medium. O. maius F3860 possessed extracellular phytase, protease, and phenol oxidase activities. The investigation indicates O. maius F3860's promise for heather seedling inoculation as an approach to increase their fitness. |
