| Autor: |
Damina SM; National Veterinary Research Institute, P.M.B.01, Vom 930103, Nigeria., Barnes DA; Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Kita 20 Nishi 10, Kita-ku, Hokkaido 001-0020, Japan., Inuwa B; National Veterinary Research Institute, P.M.B.01, Vom 930103, Nigeria., Ularamu GH; National Veterinary Research Institute, P.M.B.01, Vom 930103, Nigeria., Bello M; Department of Veterinary, Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria 810211, Nigeria., Okaiyeto OS; Department of Veterinary, Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria 810211, Nigeria., Kudi AC; Department of Veterinary, Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria 810211, Nigeria., Thapa J; Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Kita 20 Nishi 10, Kita-ku, Hokkaido 001-0020, Japan., Nakajima C; Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Kita 20 Nishi 10, Kita-ku, Hokkaido 001-0020, Japan.; International Collaboration Unit, Hokkaido University International Institute for Zoonosis Control, Kita 20 Nishi 10, Kita-ku, Hokkaido 001-0020, Japan.; Division of Research Support, Hokkaido University Institute for Vaccine Research and Development, Kita 20 Nishi 10, Kita-ku, Hokkaido 001-0020, Japan., Suzuki Y; Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Kita 20 Nishi 10, Kita-ku, Hokkaido 001-0020, Japan.; International Collaboration Unit, Hokkaido University International Institute for Zoonosis Control, Kita 20 Nishi 10, Kita-ku, Hokkaido 001-0020, Japan.; Division of Research Support, Hokkaido University Institute for Vaccine Research and Development, Kita 20 Nishi 10, Kita-ku, Hokkaido 001-0020, Japan. |
| Abstrakt: |
Bovine tuberculosis is endemic in Nigeria with control measures as provided by the laws of the country being minimally enforced mostly at the abattoirs only. This study focused on bovine tuberculosis in Adamawa and Gombe States. Tuberculosis lesions were observed in 183 of 13,688 slaughtered cattle in the regions between June and December 2020. Analysis of tissue samples resulted in 17 Mycobacterium bovis isolates, predominantly from Gombe State. Spoligotyping identified four spoligotypes, including SB0944, SB1025, SB1104, and one novel pattern. MIRU-VNTR analysis further differentiated these spoligotypes into eight profiles. All isolates belonged to the Af1 clonal complex. The study emphasises the need for broader coverage and more isolates to comprehensively understand the molecular epidemiology of bovine tuberculosis in Nigeria. To enhance research and surveillance, a cost-effective approach is proposed, utilising a discriminatory VNTR panel comprising five or nine loci. The five-locus panel consists of ETR-C, QUB26, QUB11b, MIRU04, and QUB323. Alternatively, the nine-locus panel includes ETR-A, ETR-B, QUB11a, and MIRU26. Implementing this approach would provide valuable insights into the genetic diversity of M. bovis strains in Nigeria. These findings are crucial for developing effective control measures and minimising the impact of bovine tuberculosis on both animal and human health. |
