| Autor: |
Bera M, Grushin K, Sundaram RVK, Shahanoor Z, Chatterjee A, Radhakrishnan A, Lee S, Padmanarayana M, Coleman J, Pincet F, Rothman JE, Dittman JS |
| Jazyk: |
angličtina |
| Zdroj: |
BioRxiv : the preprint server for biology [bioRxiv] 2023 Jul 16. Date of Electronic Publication: 2023 Jul 16. |
| DOI: |
10.1101/2023.07.14.549017 |
| Abstrakt: |
The critical presynaptic protein Munc13 serves numerous roles in the process of docking and priming synaptic vesicles. Here we investigate the functional significance of two distinct oligomers of the Munc13 core domain (Munc13C) comprising C1-C2B-MUN-C2C. Oligomer interface point mutations that specifically destabilized either the trimer or lateral hexamer assemblies of Munc13C disrupted vesicle docking, trans-SNARE formation, and Ca 2+ -triggered vesicle fusion in vitro and impaired neurotransmitter secretion and motor nervous system function in vivo. We suggest that a progression of oligomeric Munc13 complexes couples vesicle docking and assembly of a precise number of SNARE molecules to support rapid and high-fidelity vesicle priming. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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