Impact of an autophagy-inducing peptide on immunogenicity and protection efficacy of an adenovirus-vectored SARS-CoV-2 vaccine.

Autor:	Sayedahmed EE; Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.; Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN, USA.; Institute for Cancer Research, Purdue University, West Lafayette, IN, USA., Araújo MV; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.; Butantan Institute, São Paulo, Brazil., Silva-Pereira TT; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Chothe SK; Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA, USA., Elkashif A; Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.; Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN, USA.; Institute for Cancer Research, Purdue University, West Lafayette, IN, USA., Alhashimi M; Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.; Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN, USA.; Institute for Cancer Research, Purdue University, West Lafayette, IN, USA., Wang WC; Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.; Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN, USA.; Institute for Cancer Research, Purdue University, West Lafayette, IN, USA., Santos AP; Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.; Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN, USA.; Institute for Cancer Research, Purdue University, West Lafayette, IN, USA., Nair MS; Department of Veterinary and Biomedical Sciences, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA.; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA, USA., Gontu A; Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA, USA., Nissly R; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA, USA., Francisco de Souza Filho A; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Tavares MS; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Ayupe MC; Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Salgado CL; Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Donizetti de Oliveira Candido É; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Leal Oliveira DB; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Durigon EL; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Heinemann MB; Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, São Paulo, Brazil., Morais da Fonseca D; Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Jagannath C; Department of Pathology and Genomic Medicine, Center for Infectious Diseases and Translational Medicine, Houston Methodist Research Institute, Houston, TX, USA.; Weill Cornell Medical College, New York, NY, USA., Sá Guimarães AM; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Kuchipudi SV; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA, USA., Mittal SK; Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.; Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN, USA.; Institute for Cancer Research, Purdue University, West Lafayette, IN, USA.
Jazyk:	angličtina
Zdroj:	Molecular therapy. Methods & clinical development [Mol Ther Methods Clin Dev] 2023 Jun 27; Vol. 30, pp. 194-207. Date of Electronic Publication: 2023 Jun 27 (Print Publication: 2023).
DOI:	10.1016/j.omtm.2023.06.009
Abstrakt:	Because of continual generation of new variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is critical to design the next generation of vaccines to combat the threat posed by SARS-CoV-2 variants. We developed human adenovirus (HAd) vector-based vaccines (HAd-Spike/C5 and HAd-Spike) that express the whole Spike (S) protein of SARS-CoV-2 with or without autophagy-inducing peptide C5 (AIP-C5), respectively. Mice or golden Syrian hamsters immunized intranasally (i.n.) with HAd-Spike/C5 induced similar levels of S-specific humoral immune responses and significantly higher levels of S-specific cell-mediated immune (CMI) responses compared with HAd-Spike vaccinated groups. These results indicated that inclusion of AIP-C5 induced enhanced S-specific CMI responses and similar levels of virus-neutralizing titers against SARS-CoV-2 variants. To investigate the protection efficacy, golden Syrian hamsters immunized i.n. either with HAd-Spike/C5 or HAd-Spike were challenged with SARS-CoV-2. The lungs and nasal turbinates were collected 3, 5, 7, and 14 days post challenge. Significant reductions in morbidity, virus titers, and lung histopathological scores were observed in immunized groups compared with the mock- or empty vector-inoculated groups. Overall, slightly better protection was seen in the HAd-Spike/C5 group compared with the HAd-Spike group. Competing Interests: The authors declare no competing interests.
Databáze:	MEDLINE
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