Fibre-infused gel scaffolds guide cardiomyocyte alignment in 3D-printed ventricles.

Autor: Choi S; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA., Lee KY; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA.; Department of Integrative Bioscience and Biotechnology, Sejong University, Seoul, Republic of Korea., Kim SL; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA., MacQueen LA; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA., Chang H; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA., Zimmerman JF; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA., Jin Q; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA., Peters MM; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA., Ardoña HAM; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA.; Department of Chemical and Biomolecular Engineering, Samueli School of Engineering, University of California, Irvine, CA, USA., Liu X; Department of Cardiology, Boston Children's Hospital, Boston, MA, USA.; Fuwai Hospital Chinese Academy of Medical Sciences, Shenzhen, China., Heiler AC; Department of Bioscience, TUM School of Natural Sciences, Technische Universität München, Garching, Germany.; Center for Functional Protein Assemblies, Technische Universität München, Garching, Germany.; Center for Organoid Systems (COS), Technische Universität München, Garching, Germany., Gabardi R; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA., Richardson C; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA., Pu WT; Department of Cardiology, Boston Children's Hospital, Boston, MA, USA.; Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA., Bausch AR; Department of Bioscience, TUM School of Natural Sciences, Technische Universität München, Garching, Germany.; Center for Functional Protein Assemblies, Technische Universität München, Garching, Germany.; Center for Organoid Systems (COS), Technische Universität München, Garching, Germany.; Max Planck School Matter to Life, Max Planck Schools, Heidelberg, Germany., Parker KK; Disease Biophysics Group, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA. kkparker@g.harvard.edu.; Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA. kkparker@g.harvard.edu.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA. kkparker@g.harvard.edu.
Jazyk: angličtina
Zdroj: Nature materials [Nat Mater] 2023 Aug; Vol. 22 (8), pp. 1039-1046. Date of Electronic Publication: 2023 Jul 27.
DOI: 10.1038/s41563-023-01611-3
Abstrakt: Hydrogels are attractive materials for tissue engineering, but efforts to date have shown limited ability to produce the microstructural features necessary to promote cellular self-organization into hierarchical three-dimensional (3D) organ models. Here we develop a hydrogel ink containing prefabricated gelatin fibres to print 3D organ-level scaffolds that recapitulate the intra- and intercellular organization of the heart. The addition of prefabricated gelatin fibres to hydrogels enables the tailoring of the ink rheology, allowing for a controlled sol-gel transition to achieve precise printing of free-standing 3D structures without additional supporting materials. Shear-induced alignment of fibres during ink extrusion provides microscale geometric cues that promote the self-organization of cultured human cardiomyocytes into anisotropic muscular tissues in vitro. The resulting 3D-printed ventricle in vitro model exhibited biomimetic anisotropic electrophysiological and contractile properties.
(© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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