Potentiation of Anticancer Activity of G 2 /M Blockers by Mild Hyperthermia.

Autor:	Tagawa Y; Division of Oral and Maxillofacial Surgery, Meikai University School of Dentistry, Saitama, Japan., Sakagami H; Meikai University Research Institute of Odontology (M-RIO), Saitama, Japan; sakagami@dent.meikai.ac.jp., Tanuma SI; Meikai University Research Institute of Odontology (M-RIO), Saitama, Japan., Amano S; Meikai University Research Institute of Odontology (M-RIO), Saitama, Japan., Uota S; Meikai University Research Institute of Odontology (M-RIO), Saitama, Japan., Bandow K; Division of Biochemistry, Meikai University School of Dentistry, Saitama, Japan., Tomomura M; Division of Biochemistry, Meikai University School of Dentistry, Saitama, Japan.; Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan., Uesawa Y; Department of Medical Molecular Informatics, Meiji Pharmaceutical University, Tokyo, Japan., Takao K; Department of Pharmaceutical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Saitama, Japan., Sugita Y; Department of Pharmaceutical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Saitama, Japan., Yamamoto N; Division of Oral and Maxillofacial Surgery, Meikai University School of Dentistry, Saitama, Japan., Sakashita H; Division of Oral and Maxillofacial Surgery, Meikai University School of Dentistry, Saitama, Japan., Nakakaji R; Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan., Koizumi T; Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan., Mitsudo K; Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan., Tohnai I; Meikai University School of Health Sciences, Chiba, Japan.
Jazyk:	angličtina
Zdroj:	Anticancer research [Anticancer Res] 2023 Aug; Vol. 43 (8), pp. 3429-3439.
DOI:	10.21873/anticanres.16518
Abstrakt:	Background/aim: Hyperthermia (HT), combined with chemotherapy, has been used to treat various types of cancer. This study aimed to investigate the HT-sensitivity of malignant and non-malignant cells, and then evaluate the combination effect of docetaxel (DTX) and a newly synthesized chromone derivative (compound A) with HT. Materials and Methods: The number of viable cells was determined using the MTT method. Cell cycle distribution was analyzed using a cell sorter, and DNA fragmentation pattern was detected using agarose gel electrophoresis. Results: Among 12 cultured cells, oral squamous cell carcinoma (OSCC), especially Ca9-22 cells, and myelogenous leukemia cells showed higher sensitivity to HT than lung carcinoma and glioblastoma cell lines, while normal oral cells were the most resistant. Cytotoxicity of DTX on Ca9-22 cells was maximum at 41-42°C and 45~60 min exposure to HT. DXT, compound A, and HT induced G 2 /M arrest of Ca-22 cells. Mild HT enhanced the DTX- and compound A-induced subG 1 arrest, in a synergistic fashion. Conclusion: The combination G 2 /M blockers and mild-HT can potentially be used for the treatment of OSCC. (Copyright © 2023 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
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