High-resolution photocatalytic mapping of SARS-CoV-2 spike interactions on the cell surface.

Autor:	Datta S; Department of Biochemistry & Cell Biology, Boston University Chobanian and Avedisian School of Medicine, Boston, MA 02118, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02118, USA; Center for Network Systems Biology, Boston University, Boston, MA 02118, USA., Chen DY; Department of Biochemistry & Cell Biology, Boston University Chobanian and Avedisian School of Medicine, Boston, MA 02118, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02118, USA., Tavares AH; Department of Biochemistry & Cell Biology, Boston University Chobanian and Avedisian School of Medicine, Boston, MA 02118, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02118, USA., Reyes-Robles T; Merck Exploratory Science Center, Merck & Co., Inc, Cambridge, MA 02141, USA., Ryu KA; Merck Exploratory Science Center, Merck & Co., Inc, Cambridge, MA 02141, USA., Khan N; Department of Biochemistry & Cell Biology, Boston University Chobanian and Avedisian School of Medicine, Boston, MA 02118, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02118, USA., Bechtel TJ; Merck Exploratory Science Center, Merck & Co., Inc, Cambridge, MA 02141, USA., Bertoch JM; Merck Exploratory Science Center, Merck & Co., Inc, Cambridge, MA 02141, USA., White CH; Merck Exploratory Science Center, Merck & Co., Inc, Cambridge, MA 02141, USA., Hazuda DJ; Merck Exploratory Science Center, Merck & Co., Inc, Cambridge, MA 02141, USA; Department of Infectious Diseases and Vaccines Research, Merck & Co., Inc, West Point, PA 19486, USA., Vora KA; Department of Infectious Diseases and Vaccines Research, Merck & Co., Inc, West Point, PA 19486, USA., Hett EC; Merck Exploratory Science Center, Merck & Co., Inc, Cambridge, MA 02141, USA., Fadeyi OO; Merck Exploratory Science Center, Merck & Co., Inc, Cambridge, MA 02141, USA. Electronic address: niyi@induprotx.com., Oslund RC; Merck Exploratory Science Center, Merck & Co., Inc, Cambridge, MA 02141, USA. Electronic address: rob@induprotx.com., Emili A; Department of Biochemistry & Cell Biology, Boston University Chobanian and Avedisian School of Medicine, Boston, MA 02118, USA; Center for Network Systems Biology, Boston University, Boston, MA 02118, USA. Electronic address: emili@ohsu.edu., Saeed M; Department of Biochemistry & Cell Biology, Boston University Chobanian and Avedisian School of Medicine, Boston, MA 02118, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02118, USA. Electronic address: msaeed1@bu.edu.
Jazyk:	angličtina
Zdroj:	Cell chemical biology [Cell Chem Biol] 2023 Oct 19; Vol. 30 (10), pp. 1313-1322.e7. Date of Electronic Publication: 2023 Jul 26.
DOI:	10.1016/j.chembiol.2023.06.028
Abstrakt:	Identifying virus-host interactions on the cell surface can improve our understanding of viral entry and pathogenesis. SARS-CoV-2, the causative agent of the COVID-19 disease, uses ACE2 as a receptor to enter cells. Yet the full repertoire of cell surface proteins that contribute to viral entry is unknown. We developed a photocatalyst-based viral-host protein microenvironment mapping platform (ViraMap) to probe the molecular neighborhood of the SARS-CoV-2 spike protein on the human cell surface. Application of ViraMap to ACE2-expressing cells captured ACE2, the established co-receptor NRP1, and several novel cell surface proteins. We systematically analyzed the relevance of these candidate proteins to SARS-CoV-2 entry by knockdown and overexpression approaches in pseudovirus and authentic infection models and identified PTGFRN and EFNB1 as bona fide viral entry factors. Our results highlight additional host targets that participate in SARS-CoV-2 infection and showcase ViraMap as a powerful platform for defining viral interactions on the cell surface. Competing Interests: Declaration of interests T.R.-R., K.A.R., T.J.B., J.M.B., C.H.W., D.J.H., K.A.V., E.C.H., O.O.F., and R.C.O. were employed by Merck & Co. during the experimental planning, execution, and preparation of this manuscript. Other authors declare no competing interests. (Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze:	MEDLINE
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