A transcriptional and regulatory map of mouse somite maturation.

Autor:	Ibarra-Soria X; Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK. Electronic address: ximena.x.ibarra-soria@gsk.com., Thierion E; Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK., Mok GF; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK., Münsterberg AE; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK., Odom DT; Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK; DKFZ, Division of Regulatory Genomics and Cancer Evolution B270, Im Neunheimer Feld 280, Heidelberg, 69120, Germany. Electronic address: d.odom@dkfz-heidelberg.de., Marioni JC; Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK; European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Cambridge CB10 1SD, UK; Wellcome Sanger Institute, Wellcome Genome Campus, Cambridge CB10 1SA, UK. Electronic address: marioni@ebi.ac.uk.
Jazyk:	angličtina
Zdroj:	Developmental cell [Dev Cell] 2023 Oct 09; Vol. 58 (19), pp. 1983-1995.e7. Date of Electronic Publication: 2023 Jul 26.
DOI:	10.1016/j.devcel.2023.07.003
Abstrakt:	The mammalian body plan is shaped by rhythmic segmentation of mesoderm into somites, which are transient embryonic structures that form down each side of the neural tube. We have analyzed the genome-wide transcriptional and chromatin dynamics occurring within nascent somites, from early inception of somitogenesis to the latest stages of body plan establishment. We created matched gene expression and open chromatin maps for the three leading pairs of somites at six time points during mouse embryonic development. We show that the rate of somite differentiation accelerates as development progresses. We identified a conserved maturation program followed by all somites, but somites from more developed embryos concomitantly switch on differentiation programs from derivative cell lineages soon after segmentation. Integrated analysis of the somitic transcriptional and chromatin activities identified opposing regulatory modules controlling the onset of differentiation. Our results provide a powerful, high-resolution view of the molecular genetics underlying somitic development in mammals. Competing Interests: Declaration of interests J.C.M. has been an employee of Genentech since September 2022. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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