Effect of glycosaminoglycans on the structure and composition of articular cartilage and bone of broilers.

Autor:	Martins JMS; Department of Agricultural and Natural Sciences, Minas Gerais State University, Ituiutaba Unit, Ituiutaba, MG, Brazil., Dos Santos Neto LD; Department of Animal Science, School of Veterinary and Animal Science, Federal University of Goiás, Goiânia, GO, Brazil., Sgavioli S; Brazil University, Descalvado, SP, Brazil. Electronic address: sarahsgavioli@yahoo.com.br., Araújo ICS; Department of Animal Science, School of Veterinary Medicine, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Reis AAS; Department of Biochemistry and Molecular Biology, Institute of Biological Sciences (ICBII), Federal University of Goiás, Goiânia, GO, Brazil., Santos RS; Departament of Nature Sciences, Special Academic Unit of Human Sciences, Federal University of Goiás, Goiânia, GO, Brazil., de Araújo EG; Department of Veterinary Medicine, School of Veterinary and Animal Science, Federal University of Goiás, Goiânia, GO, Brazil., Leandro NSM; Department of Animal Science, School of Veterinary and Animal Science, Federal University of Goiás, Goiânia, GO, Brazil., Café MB; Department of Animal Science, School of Veterinary and Animal Science, Federal University of Goiás, Goiânia, GO, Brazil.
Jazyk:	angličtina
Zdroj:	Poultry science [Poult Sci] 2023 Oct; Vol. 102 (10), pp. 102916. Date of Electronic Publication: 2023 Jul 01.
DOI:	10.1016/j.psj.2023.102916
Abstrakt:	This study aimed to assess the influence of glycosaminoglycan (chondroitin and glucosamine sulfates) supplementation in the diet of broilers on the expression of matrix metallopeptidase 9 (MMP-9) and metallopeptidase inhibitor 2 (TIMP-2) genes, the synthesis of proteoglycans, collagen type II and chondrocytes, bone and cartilage macroscopy, bone mineral densitometry, bone breaking strength and mineral profile. A completely randomized design was carried out in a 3 × 3 factorial scheme (3 levels of chondroitin sulfate: 0.00, 0.05, and 0.10%; and 3 levels of glucosamine sulfate: 0.00, 0.15, and 0.30%), totaling 9 treatments. At 21 and 42 d of age, broilers were slaughtered, and tibias and femurs were collected for evaluation. There was an interaction (P < 0.05) of sulfates for the expression of MMP-9 and its inhibitor TIMP-2 in femur articular cartilage, as well as for the number of chondrocytes, collagen type II and proteoglycans in tibia articular cartilage, bone and cartilage macroscopy and mineral profile (P < 0.05), with better results obtained with the inclusion of chondroitin and/or glucosamine sulfates in the feed. In conclusion, chondroitin and glucosamine sulfates can be used in broiler diets in order to favor the development of the structure of the locomotor system (bones and joints), thus preventing locomotion problems. (Copyright © 2023. Published by Elsevier Inc.)
Databáze:	MEDLINE
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