Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care.

Autor:	Ghumra DP; Center for Aerosol Science and Engineering, Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Shetty N; Center for Aerosol Science and Engineering, Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States., McBrearty KR; Department of Neurology, Hope Center for Neurological Disease, Knight Alzheimer's Disease Research Center, Washington University, St. Louis, Missouri 63110, United States., Puthussery JV; Center for Aerosol Science and Engineering, Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Sumlin BJ; Center for Aerosol Science and Engineering, Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Gardiner WD; Department of Neurology, Hope Center for Neurological Disease, Knight Alzheimer's Disease Research Center, Washington University, St. Louis, Missouri 63110, United States., Doherty BM; Department of Neurology, Hope Center for Neurological Disease, Knight Alzheimer's Disease Research Center, Washington University, St. Louis, Missouri 63110, United States., Magrecki JP; Department of Neurology, Hope Center for Neurological Disease, Knight Alzheimer's Disease Research Center, Washington University, St. Louis, Missouri 63110, United States., Brody DL; National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892, United States.; Department of Neurology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, United States., Esparza TJ; National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892, United States., O'Halloran JA; Department of Medicine, Washington University, St. Louis, Missouri 63110, United States., Presti RM; Department of Medicine, Washington University, St. Louis, Missouri 63110, United States., Bricker TL; Department of Medicine, Washington University, St. Louis, Missouri 63110, United States.; Departments Molecular Microbiology, and Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110, United States., Boon ACM; Department of Medicine, Washington University, St. Louis, Missouri 63110, United States.; Departments Molecular Microbiology, and Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110, United States., Yuede CM; Department of Psychiatry, Washington University School of Medicine, Campus Box 8134, 660 South Euclid Avenue, St. Louis, Missouri 63110, United States., Cirrito JR; Department of Neurology, Hope Center for Neurological Disease, Knight Alzheimer's Disease Research Center, Washington University, St. Louis, Missouri 63110, United States., Chakrabarty RK; Center for Aerosol Science and Engineering, Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States.
Jazyk:	angličtina
Zdroj:	ACS sensors [ACS Sens] 2023 Aug 25; Vol. 8 (8), pp. 3023-3031. Date of Electronic Publication: 2023 Jul 27.
DOI:	10.1021/acssensors.3c00512
Abstrakt:	Airborne transmission via virus-laden aerosols is a dominant route for the transmission of respiratory diseases, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Direct, non-invasive screening of respiratory virus aerosols in patients has been a long-standing technical challenge. Here, we introduce a point-of-care testing platform that directly detects SARS-CoV-2 aerosols in as little as two exhaled breaths of patients and provides results in under 60 s. It integrates a hand-held breath aerosol collector and a llama-derived, SARS-CoV-2 spike-protein specific nanobody bound to an ultrasensitive micro-immunoelectrode biosensor, which detects the oxidation of tyrosine amino acids present in SARS-CoV-2 viral particles. Laboratory and clinical trial results were within 20% of those obtained using standard testing methods. Importantly, the electrochemical biosensor directly detects the virus itself, as opposed to a surrogate or signature of the virus, and is sensitive to as little as 10 viral particles in a sample. Our platform holds the potential to be adapted for multiplexed detection of different respiratory viruses. It provides a rapid and non-invasive alternative to conventional viral diagnostics.
Databáze:	MEDLINE
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