New small-molecule alcohol synthesis by breaking the space limitation of the "aromatic cage" in Pseudomonas sp. AK1 BBOX.

Autor:	Xu Z; School of Pharmacy, Shanxi Medical University, Taiyuan, Shanxi Province 030001, China. shurongban@sxmu.edu.cn., Mo Y; School of Pharmacy, Shanxi Medical University, Taiyuan, Shanxi Province 030001, China. shurongban@sxmu.edu.cn., Li Z; School of Pharmacy, Shanxi Medical University, Taiyuan, Shanxi Province 030001, China. shurongban@sxmu.edu.cn., Ban S; School of Pharmacy, Shanxi Medical University, Taiyuan, Shanxi Province 030001, China. shurongban@sxmu.edu.cn., Song H; College of Chemistry & Molecular Science, Wuhan University, Wuhan, Hubei Province 430072, China. hengsong@whu.edu.cn.; Wuhan University Shenzhen Research Institute, Shenzhen, Guangdong Province 518000, China.
Jazyk:	angličtina
Zdroj:	Organic & biomolecular chemistry [Org Biomol Chem] 2023 Aug 09; Vol. 21 (31), pp. 6397-6404. Date of Electronic Publication: 2023 Aug 09.
DOI:	10.1039/d3ob00830d
Abstrakt:	Fe(II)/2OG-dependent oxygenase γ-butyrobetaine hydroxylase (BBOX) stereoselectively hydroxylates inactive C-H bonds and produces L-carnitine. It has potential applications in the biosynthesis of L-carnitine and the synthesis of other small molecule alcohols. In this paper, we systematically explore the substrate range of Pseudomonas sp. AK1 BBOX (psBBOX), with emphasis on the quaternary ammonium portion of γ-butyrobetaine (γ-BB). The space limitation of the "aromatic cage" in psBBOX in the hydroxylation of large quaternary ammonium analogues was studied, and the role of four aromatic amino acid residues in the substrate binding mode was analyzed. Consequently, the F188A mutant was developed with the ability to hydroxylate cyclic quaternary ammonium analogues and generate new alcohol compounds by breaking the limitation of the "aromatic cage".
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu