Investigation of refractive index dynamics during in vitro embryo development using off-axis digital holographic microscopy.

Autor:	Dwapanyin GO; SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, Fife, United Kingdom., Chow DJX; Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, Australia.; Australian Research Council Centre of Excellence for Nanoscale Biophotonics, The University of Adelaide, Adelaide, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia., Tan TCY; Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, Australia.; Australian Research Council Centre of Excellence for Nanoscale Biophotonics, The University of Adelaide, Adelaide, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia., Dubost NS; SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, Fife, United Kingdom., Morizet JM; SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, Fife, United Kingdom., Dunning KR; Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, Australia.; Australian Research Council Centre of Excellence for Nanoscale Biophotonics, The University of Adelaide, Adelaide, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia.; kylie.dunning@adelaide.edu.au., Dholakia K; SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, Fife, United Kingdom.; School of Biological Sciences, The University of Adelaide, Adelaide, Australia.; Centre of Light for Life, The University of Adelaide, Adelaide, Australia.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2023 Jun 13; Vol. 14 (7), pp. 3327-3342. Date of Electronic Publication: 2023 Jun 13 (Print Publication: 2023).
DOI:	10.1364/BOE.492292
Abstrakt:	Embryo quality is a crucial factor affecting live birth outcomes. However, an accurate diagnostic for embryo quality remains elusive in the in vitro fertilization clinic. Determining physical parameters of the embryo may offer key information for this purpose. Here, we demonstrate that digital holographic microscopy (DHM) can rapidly and non-invasively assess the refractive index of mouse embryos. Murine embryos were cultured in either low- or high-lipid containing media and digital holograms recorded at various stages of development. The phase of the recorded hologram was numerically retrieved, from which the refractive index of the embryo was calculated. We showed that DHM can detect spatio-temporal changes in refractive index during embryo development that are reflective of its lipid content. As accumulation of intracellular lipid is known to compromise embryo health, DHM may prove beneficial in developing an accurate, non-invasive, multimodal diagnostic. Competing Interests: The authors declare that no competing interests exist. (Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.)
Databáze:	MEDLINE
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