Evaluation of the effects of the recommended oral dose of diflubenzuron on bovine sperm and oocyte quality using CASA and OPU-IVEP.

Autor:	Xavier MC; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF, Brazil.; Bio Biotecnologia da Reprodução Animal, Brasília, DF, Brazil., Martins LP; Programa de Pós-Graduação em Biologia Animal, Universidade de Brasília, Brasília, DF, Brazil., Moura RM; Programa de Pós-Graduação em Biologia Animal, Universidade de Brasília, Brasília, DF, Brazil., Morais DF; Departamento de Medicina Veterinária, Centro Universitário de Brasília, Brasília, DF, Brazil., Barbosa JVL; Departamento de Medicina Veterinária, Centro Universitário de Brasília, Brasília, DF, Brazil., Figueiredo RA; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil., Peixer MAS; Bio Biotecnologia da Reprodução Animal, Brasília, DF, Brazil., de Andrade RV; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF, Brazil., Viana JHM; Programa de Pós-Graduação em Biologia Animal, Universidade de Brasília, Brasília, DF, Brazil.; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2023 Jul 11; Vol. 10, pp. 1215722. Date of Electronic Publication: 2023 Jul 11 (Print Publication: 2023).
DOI:	10.3389/fvets.2023.1215722
Abstrakt:	Introduction: The aim of this study was to evaluate potential effects of diflubenzuron on the production and quality of gametes, and on in vitro embryo production (IVEP) outcomes, in cattle. Methods: Two experiments were performed, the first to evaluate effects on semen, and the second on cumulus -oocyte complexes (COC) and on IVEP. Nelore ( Bos taurus indicus ) bulls ( n = 14) or heifers ( n = 16) were allocated into control (CG) or treatment (DIF) groups. All groups received a mineral mix supplement added (DIF) or not (CG) with diflubenzuron (30 mg/head/day), during 8 weeks. Animals were weighed and blood samples were collected throughout the experimental period. Every other week, bulls were subjected to semen collection and heifers to transvaginal ultrasound-guided follicle aspiration sessions. Semen underwent physical and morphological evaluation, and samples were stored for further computer-assisted sperm analysis. The COC recovered were evaluated according to morphology and those classified as viable were sent to an IVEP laboratory. Results: Diflubenzuron had no effect ( P > 0.05) on average body weight or in any blood hematological or biochemical endpoints, regardless of gender. In experiment 1, there was no difference ( P > 0.05) between DIF and CG groups for sperm concentration, morphology, or kinetics. In experiment 2, there was also no effect of diflubenzuron on the number of total, viable, or grade I oocytes, as well as on cleavage or blastocyst rates ( P > 0.05). Discussion: In summary, the oral administration of diflubenzuron, within the recommended dose, has no short-term negative effects on sperm production and quality or on oocyte yield and developmental potential in vitro , in cattle. Competing Interests: MX and MP were employed by Bio Biotecnologia da Reprodução Animal. RF and JV were employed by Embrapa Recursos Genéticos e Biotecnologia. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Xavier, Martins, Moura, Morais, Barbosa, Figueiredo, Peixer, Andrade and Viana.)
Databáze:	MEDLINE
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