Induction of tomato plant biochemical immune responses by the synthesized zinc oxide nanoparticles against wilt-induced Fusarium oxysporum.

Autor: Bouqellah NA; Biology Department, Science College, Taibah University, Al Madinah Al Munawwarah, 42317-8599, Saudi Arabia. Nahla.B@hotmail.com., El-Sayyad GS; Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, sixth of October City, Giza, Egypt. Gharieb.Elsayyad@gu.edu.eg.; Department of Microbiology and Immunology, Faculty of Pharmacy, Galala University, New Galala City, Suez, Egypt. Gharieb.Elsayyad@gu.edu.eg.; Drug Microbiology Lab., Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt. Gharieb.Elsayyad@gu.edu.eg., Attia MS; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Jazyk: angličtina
Zdroj: International microbiology : the official journal of the Spanish Society for Microbiology [Int Microbiol] 2024 Apr; Vol. 27 (2), pp. 435-448. Date of Electronic Publication: 2023 Jul 25.
DOI: 10.1007/s10123-023-00404-7
Abstrakt: The current study used zinc oxide nanoparticles (ZnO-NPs) to protect the tomato plant against Fusarium wilt. Gamma rays were used to synthesize ZnO-NPs, and the designed ZnO-NPs were characterized using high-resolution transmission electron microscopy (HRTEM), scanning electron microscope (SEM), dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDX), and ultraviolet-visible (UV-Vis.) spectroscopy. We found that the 20 kGy dose is the most effective for ZnO-NPs synthesis, with the highest O.D. = 1.65 (diluted 3 times) at 400 nm. The scale of ZnO-NPs ranged from 10.45 to 75.25 nm with an average diameter of 40.20 nm. The results showed that the designed ZnO-NPs showed promising activity as a potent inducer of plant physiological immunity against Fusarium wilt disease. Likewise, ZnO-NPs significantly reduced the wilt disease symptoms incidence by 28.57% and high protection by 67.99% against F. oxysporum. Additionally, infected tomato plants treated with ZnO-NPs show improved shoot length (44.71%), root length (40.0%), number of leaves (60.0 %), chlorophyll a (36.93%), chlorophyll b (16.46%), and carotenoids (21.87%) versus infected plants. Notably, in the treatment of tomato seedlings, the beneficial effects of ZnO-NPs extended to increase not only in osmolyte contents but also total phenol contents in comparison with control plants. In conclusion, the designed ZnO-NPs can control Fusarium wilt disease and improve and develop biochemical compounds responsible for defense against fusarial infection.
(© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
Externí odkaz: