Application of infrared matrix-assisted laser desorption electrospray ionization mass spectrometry for morphine imaging in brain tissue.

Autor: Desyaterik Y; Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. dessiat@email.unc.edu., Mwangi JN; Shattuck Labs, Durham, NC, USA., McRae M; School of Medicine, University of Virginia, Charlottesville, VA, USA., Jones AM; School of Pharmacy, Virginia Commonwealth University, Richmond, VA, USA., Kashuba ADM; Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Rosen EP; Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2023 Sep; Vol. 415 (23), pp. 5809-5817. Date of Electronic Publication: 2023 Jul 25.
DOI: 10.1007/s00216-023-04861-x
Abstrakt: Here, we present a method developed for the analysis of spatial distributions of morphine in mouse brain tissue using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) coupled to a Q Exactive Plus mass spectrometer. The method is also capable of evaluating spatial distributions of the antiretroviral drug abacavir. To maximize sensitivity to morphine, we analyze various Orbitrap mass spectrometry acquisition modes utilizing signal abundance and frequency of detection as evaluation criteria. We demonstrate detection of morphine in mouse brain and establish that the selected ion monitoring mode provides 2.5 times higher sensitivity than the full-scan mode. We find that distributions of morphine and abacavir are highly correlated with the Pearson correlation coefficient R = 0.87. Calibration showed that instrument response is linear up to 40 pg/mm 2 (3.8 μg/g of tissue).
(© 2023. The Author(s).)
Databáze: MEDLINE
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