A Rapidly Responsive Sensor for Wireless Detection of Early and Mature Microbial Biofilms.

Autor: Shafaat A; Department of Biomedical Science, Faculty of Health and Society, Malmö University, 20506, Malmö, Sweden.; Biofilms - Research Center for Biointerfaces, Malmö University, 20506, Malmö, Sweden., Gonzalez-Martinez JF; Department of Applied Physics, Universidad Politécnica de Cartagena, 30202, Cartagena, Spain., Silva WO; Institute of Systems Engineering, HES-SO Valais-Wallis, 1950, Sion, Switzerland., Lesch A; Department of Industrial Chemistry 'Toso Montanari', University of Bologna, Viale del Risorgimento 4, 40136, Bologna, Italy., Nagar B; Laboratory of Physical and Analytical Electrochemistry, École Polytechnique Fédérale de Lausanne (EPFL) Valais Wallis, 1950, Sion, Switzerland., Lopes da Silva Z; Department of Oral Biology, Faculty of Odontology, Malmö University, 20506, Malmö, Sweden., Neilands J; Department of Oral Biology, Faculty of Odontology, Malmö University, 20506, Malmö, Sweden., Sotres J; Department of Biomedical Science, Faculty of Health and Society, Malmö University, 20506, Malmö, Sweden.; Biofilms - Research Center for Biointerfaces, Malmö University, 20506, Malmö, Sweden., Björklund S; Department of Biomedical Science, Faculty of Health and Society, Malmö University, 20506, Malmö, Sweden.; Biofilms - Research Center for Biointerfaces, Malmö University, 20506, Malmö, Sweden., Girault H; Laboratory of Physical and Analytical Electrochemistry, École Polytechnique Fédérale de Lausanne (EPFL) Valais Wallis, 1950, Sion, Switzerland., Ruzgas T; Department of Biomedical Science, Faculty of Health and Society, Malmö University, 20506, Malmö, Sweden.; Biofilms - Research Center for Biointerfaces, Malmö University, 20506, Malmö, Sweden.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2023 Oct 02; Vol. 62 (40), pp. e202308181. Date of Electronic Publication: 2023 Aug 25.
DOI: 10.1002/anie.202308181
Abstrakt: Biofilm-associated infections, which are able to resist antibiotics, pose a significant challenge in clinical treatments. Such infections have been linked to various medical conditions, including chronic wounds and implant-associated infections, making them a major public-health concern. Early-detection of biofilm formation offers significant advantages in mitigating adverse effects caused by biofilms. In this work, we aim to explore the feasibility of employing a novel wireless sensor for tracking both early-stage and matured-biofilms formed by the medically relevant bacteria Staphylococcus aureus and Pseudomonas aeruginosa. The sensor utilizes electrochemical reduction of an AgCl layer bridging two silver legs made by inkjet-printing, forming a part of near-field-communication tag antenna. The antenna is interfaced with a carbon cloth designed to promote the growth of microorganisms, thereby serving as an electron source for reduction of the resistive AgCl into a highly-conductive Ag bridge. The AgCl-Ag transformation significantly alters the impedance of the antenna, facilitating wireless identification of an endpoint caused by microbial growth. To the best of our knowledge, this study for the first time presents the evidence showcasing that electrons released through the actions of bacteria can be harnessed to convert AgCl to Ag, thus enabling the wireless, battery-less, and chip-less early-detection of biofilm formation.
(© 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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