| Autor: |
Schültke E; Department of Radiooncology, Rostock University Medical Center, 18059 Rostock, Germany., Pinzer BR; Laboratory for Optical 3D Metrology and Computer Vision, University of Applied Sciences Kempten, 87435 Kempten, Germany., Stampanoni M; Paul Scherrer Institute, 5232 Villigen, Switzerland., Harsan L; ICube Laboratory (Engineering Science, Computer Science and Imaging Laboratory) and Biophysics and Nuclear Medicine Department, Faculty of Medicine, University of Strasbourg, 67412 Strasbourg, France., Döbrössy M; Department of Stereotactic and Functional Neurosurgery, Freiburg University Medical Center, 79106 Freiburg, Germany.; Faculty of Biology, University of Freiburg, 79106 Freiburg, Germany. |
| Abstrakt: |
High-resolution imaging in small animal models of neurologic disease is a technical challenge. In a pilot project, we have explored a non-destructive synchrotron imaging technique for the 3D visualization of intracerebral tissue transplants in a well-established small animal model of Huntington's disease. Four adult female Sprague Dawley rats each received injections of 0.12 M quinolinic acid (QA) into two target positions in the left striatum, thus creating unilateral left-sided striatal lesions similar to those frequently seen in patients suffering from Huntington's disease. One week after lesioning, the animals received transplants prepared from whole ganglionic eminences (wGEs) obtained from 13- to 14-day-old rat embryos. Of the four lesioned animals, three received transplants of GNP-loaded cells and one animal received a transplant of naïve cells, serving as control. Post-mortem synchrotron-based microCT was used to obtain images of the neurotransplants. The images obtained of GNP-loaded tissue transplants at the synchrotron corresponded in size and shape to the histological images of transplants developed from naïve cells. Thus, we conclude that non-destructive synchrotron imaging techniques such as phase-contrast imaging are suitable to obtain high-resolution images of GNP-loaded tissue transplants. |
