Redirecting the JAK-STAT signal blocks the SARS-CoV-2 replication.

Autor: Augustine G; Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research-Central Leather Research Institute (CSIR-CLRI), Chennai, India.; Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon, USA., Sisila V; Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research-Central Leather Research Institute (CSIR-CLRI), Chennai, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India., Indhu M; Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research-Central Leather Research Institute (CSIR-CLRI), Chennai, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India., Gupta D; Council of Scientific and Industrial Research-Centre for Cellular and Molecular Biology (CSIR-CCMB), Hyderabad, India., Tandel D; Council of Scientific and Industrial Research-Centre for Cellular and Molecular Biology (CSIR-CCMB), Hyderabad, India., Harshan KH; Council of Scientific and Industrial Research-Centre for Cellular and Molecular Biology (CSIR-CCMB), Hyderabad, India., Shanmugam G; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.; Department of Bio-Organic Chemistry, Council of Scientific and Industrial Research-Central Leather Research Institute (CSIR-CLRI), Chennai, India., Padmapriya P; Department of Virology, King Institute of Preventive Medicine and Research, Chennai, India., Sivasubramanian S; Department of Virology, King Institute of Preventive Medicine and Research, Chennai, India., Kaveri K; Department of Virology, King Institute of Preventive Medicine and Research, Chennai, India., Ramudu KN; Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research-Central Leather Research Institute (CSIR-CLRI), Chennai, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India., Ayyadurai N; Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research-Central Leather Research Institute (CSIR-CLRI), Chennai, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.; Council of Scientific and Industrial Research-Centre for Cellular and Molecular Biology (CSIR-CCMB), Hyderabad, India.
Jazyk: angličtina
Zdroj: Journal of medical virology [J Med Virol] 2023 Jul; Vol. 95 (7), pp. e28965.
DOI: 10.1002/jmv.28965
Abstrakt: The distinct disease progression patterns of severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2) indicate diverse host immune responses. SARS-CoV-2 severely impairs type I interferon (IFN) cell signaling, resulting in uncontrolled late-phase lung damage in patients. For better pharmacological properties, cytokine modifications may sometimes result in a loss of biological activity against the virus. Here, we employed the genetic code expansion and engineered IFN-β, a phase II clinical cytokine with 3-amino tyrosine (IFN-β-A) that reactivates STAT2 expression in virus-infected human cells through JAK/STAT cell signaling without affecting signal activation and serum half-life. This study identified that genetically encoded IFN-β-A might stabilize the protein-receptor complex and trigger JAK-STAT cell signaling, which is a promising modality for controlling SARS-CoV-2 infection.
(© 2023 Wiley Periodicals LLC.)
Databáze: MEDLINE
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