The protective role of sulforaphane and Homer1a in retinal ischemia-reperfusion injury: Unraveling the neuroprotective interplay.

Autor: Saadh MJ; Faculty of Pharmacy, Middle East University, Amman 11831, Jordan; Applied Science Research Center, Applied Science Private University, Amman 11152, Jordan., Castillo-Acobo RY; Universidad Nacional de San Agustin de Arequipa, Arequipa, Peru., Baher H; Department of Radiology and Ultrasonography Techniques, College of Medical Techniques, Al-Farahidi University, Baghdad, Iraq., Narayanan J; Department of Pharmacology, SRM College of Pharmacy, SRMIST, Tamil Nadu, India., Palacios Garay JP; Universidad Nacional Mayor de San Marcos, Lima, Peru., Yamaguchi MNV; Universidad de San Martín de Porres, Lima, Peru., Arias-Gonzáles JL; Department of Social Sciences, Faculty of Social Studies, University of British Columbia, BC, Canada., Cotrina-Aliaga JC; Universidad Peruana de los Andes, Huancayo, Peru., Akram SV; Uttaranchal Institute of Technology, Division of research and Innovation, Uttaranchal University, Dehradun, India., Lakshmaiya N; Department of Mechanical Engineering, Saveetha School of Engineering, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India., Amin AH; Zoology Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt., Mohany M; Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 11451, Saudi Arabia., Al-Rejaie SS; Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh 11451, Saudi Arabia., Ahsan M; Department of Measurements and Control Systems, Silesian University of Technology, Gliwice, 44-100, Poland; Joint Doctoral School, Silesian University of Technology, Akademicka 2A, Gliwice, 44-100, Poland. Electronic address: Muhammad.Ahsan@polsl.pl., Bahrami A; Department of Cell Biology, Tuebingen University, Tuebingen, Germany; Biomedical Center for Systems Biology Science Munich, Ludwig-Maximilians-University, Munich, Germany. Electronic address: A.Bahrami@ut.ac.ir., Akhavan-Sigari R; Department of Health Care Management and Clinical Research, Collegium Humanum Warsaw, Poland; Department of Neurosurgery, University Medical Center Tuebingen, Germany.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2023 Sep 15; Vol. 329, pp. 121968. Date of Electronic Publication: 2023 Jul 22.
DOI: 10.1016/j.lfs.2023.121968
Abstrakt: Aims: Retinal ischemia/reperfusion (I/R) injury is a common pathological basis for various ophthalmic diseases. This study aimed to investigate the potential of sulforaphane (SFN) and Homer1a in regulating cell apoptosis induced by retinal I/R injury and to explore the underlying regulatory mechanism between them.
Materials and Methods: In in vivo experiments, C57BL/6J mice and Homer1 flox/- /Homer1a +/- /Nestin-Cre +/- mice were used to construct retinal I/R injury models. In vitro experiments utilized the oxygen-glucose deprivation-reperfusion (OGD/R) injury model with primary retinal ganglion cells (RGCs). The effects of Homer1a and SFN on cell apoptosis were observed through pathological analyses, flow cytometry, and visual electrophysiological assessments.
Key Findings: We discovered that after OGD/R injury, apoptosis of RGCs and intracellular Ca 2+ activity significantly increased. However, these changes were reversed upon the addition of SFN, and similar observations were reproduced in in vivo studies. Furthermore, both in vivo and in vitro studies confirmed the upregulation of Homer1a after I/R, which could be further enhanced by the administration of SFN. Moreover, upregulation of Homer1a resulted in a reduction in cell apoptosis and pro-apoptotic proteins, while downregulation of Homer1a had the opposite effect. Flash visual evoked potential, oscillatory potentials, and escape latency measurements in mice supported these findings. Furthermore, the addition of SFN strengthened the neuroprotective effects in the OGD/R + H + group but weakened them in Homer1 flox/- /Homer1a +/- /Nestin-Cre +/- mice.
Significance: These results indicate that Homer1a plays a significant role in the therapeutic potential of sulforaphane for retinal I/R injury, thereby providing a theoretical basis for clinical treatment.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier Inc.)
Databáze: MEDLINE
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