More insights about genomic population structure of Mycobacterium avium subspecies paratuberculosis (Map) from multiple hosts in west and central provinces of Iran using a boosted genotyping approach.

Autor: Najafpour R; Department of Microbiology, Faculty of Basic Science, Qom Branch, Islamic Azad University, Qom, Iran., Zolfaghari MR; Department of Microbiology, Faculty of Basic Science, Qom Branch, Islamic Azad University, Qom, Iran. Electronic address: Mreza.zolfaghary@gmail.com., Mosavari N; Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bovine Tuberculosis Laboratory, Tuberculin and Mallein Research & Production Department, Karaj, Iran., Nazari R; Department of Microbiology, Faculty of Basic Science, Qom Branch, Islamic Azad University, Qom, Iran., Tadayon K; Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Veterinary, Aerobic Bacteria Vaccines Department, Karaj, Iran. Electronic address: mmb093@gmail.com.
Jazyk: angličtina
Zdroj: Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2023 Sep; Vol. 100, pp. 101912. Date of Electronic Publication: 2022 Nov 12.
DOI: 10.1016/j.cimid.2022.101912
Abstrakt: To investigate the population genetic of Mycobacterium avium subsp. paratuberculosis (Map) in Iran, Mycobacterial Interspersed Repetitive Units (MIRUs) and Multi Locus Short Sequence Repeat (MLSSR) system were employed. Numerous genotypes by MIRU (N = 11) and MLSSR (N = 9) methods bearing discriminatory indices of 0.90 and 0.79 respectively, were obtained. Browsing the INRA-Nouzilly list (http://mac-inmv.tours.inra.fr/) detected 3 of the found patterns as new types. Some loci either MIRU-VNTR or SSR proved more polymorphic and therefore are recommended to be applied in priority for strain typing in the Iranian environment. While identical MIRU-VNTR or MLSSR patterns were detected among different conspecifics and geographical locations, dissimilar types were also observed at the same farms an indication of coexistence of Map strains within one herd. We suggest extension of the genotyping work described here to include more endogenous isolates in order to better analysis of transmission and virulence in epidemiology and control of paratuberculosis.
Competing Interests: Declaration of Competing Interest The authors declare that there are no known personal or competing financial relationships of any sort to influence the work presented in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: