| Autor: |
Marini PVB; Medical Microbiology Laboratory, Department of Basic Health Sciences, State University of Maringá, Maringá, Brazil., Tavares ER; Laboratory of Molecular Biology of Microorganisms, Department of Microbiology, State University of Londrina, Londrina, Brazil., Motter CW; Medical Microbiology Laboratory, Department of Basic Health Sciences, State University of Maringá, Maringá, Brazil., Migliorini LB; Albert Einstein Research and Education Institute, Hospital Israelita Albert Einstein, São Paulo, Brazil., de Sales RO; Albert Einstein Research and Education Institute, Hospital Israelita Albert Einstein, São Paulo, Brazil., Fedrigo NH; Medical Microbiology Laboratory, Department of Basic Health Sciences, State University of Maringá, Maringá, Brazil., Shinohara DR; Medical Microbiology Laboratory, Department of Basic Health Sciences, State University of Maringá, Maringá, Brazil., Hungria M; Laboratory of Soy Biotechnology, Embrapa Soja, Londrina, Brazil., Yamada-Ogatta SF; Laboratory of Molecular Biology of Microorganisms, Department of Microbiology, State University of Londrina, Londrina, Brazil., Tognim MCB; Medical Microbiology Laboratory, Department of Basic Health Sciences, State University of Maringá, Maringá, Brazil. |
| Abstrakt: |
Raoultella planticola harboring genes that confer resistance to antimicrobials, such as carbapenems, have been associated with severe infections in immunocompromised patients. In this study, we reported the first whole genome sequence of a Brazilian isolate of R. planticola and the genomic context of antibiotic resistance markers. By whole-genome sequencing (WGS) of a carbapenem-resistant R. planticola isolate, RpHUM1, we found 23 resistance-encoding genes belonging to 9 classes of antibiotics (aminoglycosides, β-lactams, fluoroquinolones, fosfomycin, macrolides, phenicols, sulfonamides, tetracycline, and diaminopyrimidine derivatives) and 3 plasmids (RpHUM1pEaer-4382s, RpHUM1_pFDAARGOS_440, and RpHUM1pRSF1010). This isolate coharbored the genes bla KPC-2 , which is carried by the plasmid RpHUM1pEaer-4382s, and bla NDM-1 and bla CTX-M-15 all located in the accessory genome. In addition, these genes were associated with, at least, one mobile genetic element. This comprehensive knowledge is of great importance for implementation of control measures to prevent the rapid dissemination of this neglected microorganism and their genetic resistance background. |
