| Autor: |
Raval YS; Department of Laboratory Medicine and Division of Clinical Microbiology, Mayo Clinic, Rochester, MN., Fleming D; Department of Laboratory Medicine and Division of Clinical Microbiology, Mayo Clinic, Rochester, MN., Mohamed A; The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA., Karau MJ; Department of Laboratory Medicine and Division of Clinical Microbiology, Mayo Clinic, Rochester, MN., Mandrekar JN; Department of Quantitative Health Sciences, Mayo Clinic, Rochester, MN., Schuetz AN; Department of Laboratory Medicine and Division of Clinical Microbiology, Mayo Clinic, Rochester, MN., Greenwood Quaintance KE; Department of Laboratory Medicine and Division of Clinical Microbiology, Mayo Clinic, Rochester, MN., Beyenal H; The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA., Patel R; Department of Laboratory Medicine and Division of Clinical Microbiology, Mayo Clinic, Rochester, MN.; Division of Public Health, Infectious Diseases, and Occupational Medicine, Mayo Clinic, Rochester, MN. |
| Abstrakt: |
Biofilms formed by antibiotic-resistant bacteria in wound beds present unique challenges in terms of treating wound infections. In this work, the in vivo activity of a novel electrochemical bandage (e-bandage) composed of carbon fabric and controlled by a wearable potentiostat, designed to continuously deliver low amounts of hydrogen peroxide (H 2 O 2 ) was evaluated against methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant Pseudomonas aeruginosa (MDR-PA) and mixed-species (MRSA and MDR-PA) wound infections. Wounds created on Swiss Webster mice were infected with the above-named bacteria and biofilms allowed to establish on wound beds for 3 days. e-Bandages, which electrochemically reduce dissolved oxygen to H 2 O 2 when polarized at -0.6 V Ag/AgCl , were placed atop the infected wound bed and polarized continuously for 48 hours. Polarized e-bandage treatment resulted in significant reductions (p <0.001) of both mono-species and mixed-species wound infections. After e-bandage treatment, electron microscopy showed degradation of bacterial cells, and histopathology showed no obvious alteration to the inflammatory host response. Blood biochemistries showed no abnormalities. Taken all together, results of this work suggest that the described H 2 O 2 -producing e-bandage can effectively reduce in vivo MRSA, MDR-PA and mixed-species wound biofilms, and should be further developed as a potential antibiotic-free strategy for treatment of wound infections. |
Plný text