Establishment of ex vivo calibration curve for X-ray induced "dicentric + ring" and micronuclei in human peripheral lymphocytes for biodosimetry during radiological emergencies, and validation with dose blinded samples.
| Autor: | Vijayalakshmi J; Department of Human Genetics, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, India., Chaurasia RK; Radiological Physics and Advisory Division, Bhabha Atomic Research Centre (BARC), Mumbai, India.; Homi Bhabha National Institute (HBNI), Mumbai, India., Srinivas KS; Department of Human Genetics, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, India., Vijayalakshmi K; Department of Human Genetics, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, India., Paul SFD; Department of Human Genetics, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, India., Bhat NN; Radiological Physics and Advisory Division, Bhabha Atomic Research Centre (BARC), Mumbai, India.; Homi Bhabha National Institute (HBNI), Mumbai, India., Sapra BK; Radiological Physics and Advisory Division, Bhabha Atomic Research Centre (BARC), Mumbai, India.; Homi Bhabha National Institute (HBNI), Mumbai, India. |
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| Jazyk: | angličtina |
| Zdroj: | Heliyon [Heliyon] 2023 Jun 07; Vol. 9 (6), pp. e17068. Date of Electronic Publication: 2023 Jun 07 (Print Publication: 2023). |
| DOI: | 10.1016/j.heliyon.2023.e17068 |
| Abstrakt: | In the modern developing society, application of radiation has increased extensively. With significant improvement in the radiation protection practices, exposure to human could be minimized substantially, but cannot be avoided completely. Assessment of exposure is essential for regulatory decision and medical management as applicable. Until now, cytogenetic changes have served as surrogate marker of radiation exposure and have been extensively employed for biological dose estimation of various planned and unplanned exposures. Dicentric Chromosomal Aberration (DCA) is radiation specific and is considered as gold standard, micronucleus is not very specific to radiation and is considered as an alternative method for biodosimetry. In this study dose response curves were generated for X-ray induced "dicentric + ring" and micronuclei, in lymphocytes of three healthy volunteers [2 females (age 22, 23 years) and 1 male (24 year)]. The blood samples were irradiated with X-ray using LINAC (energy 6 MV, dose rate 6 Gy/min), in the dose range of 0-5Gy. Irradiated blood samples were cultured and processed to harvest metaphases, as per standard procedures recommended by International Atomic Energy Agency. Pooled data obtained from all the three volunteers, were in agreement with Poisson distribution for "dicentric + ring", however over dispersion was observed for micronuclei. Data ("dicentric + ring" and micronuclei) were fitted by linear quadratic model of the expression Y[bond, double bond]C + αD + βD 2 using Dose Estimate software, version 5.2. The data fit has resulted in linear coefficient α = 0.0006 (±0.0068) "dicentric + ring" cell -1 Gy -1 and quadratic coefficient β = 0.0619 (±0.0043) "dicentric + ring" cell -1 Gy -2 for "dicentric + ring" and linear coefficient α = 0.0459 ± (0.0038) micronuclei cell -1 Gy -1 and quadratic coefficient β = 0.0185 ± (0.0010) micronuclei cell -1 Gy -2 for micronuclei, respectively. Background frequencies for "dicentric + ring" and micronuclei were 0.0006 ± 0.0004 and 0.0077 ± 0.0012 cell -1 , respectively. Established curves were validated, by reconstructing the doses of 8 dose blinded samples (4 by DCA and 4 by CBMN) using coefficients generated here. Estimated doses were within the variation of 0.9-16% for "dicentric + ring" and 21.7-31.2% for micronuclei respectively. These established curves have potential to be employed for biodosimetry of occupational, clinical and accidental exposures, for initial triage and medical management. Competing Interests: Authors declare no known conflict of interest.Authors declare that, they do not have any conflict of interests. (© 2023 Published by Elsevier Ltd.) |
| Databáze: | MEDLINE |
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