Effect of dissolved metabolites of the dinoflagellate Gymnodinium catenatum (Graham, 1943) on the white shrimp Litopenaeus vannamei (Boone, 1931): A histological study.
| Autor: | Flores-Chavarria AM; Centro de Investigaciones Biológicas del Noroeste, Av. Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita Sur, C.P. 23096, La Paz, B.C.S., Mexico., Rodríguez-Jaramillo C; Centro de Investigaciones Biológicas del Noroeste, Av. Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita Sur, C.P. 23096, La Paz, B.C.S., Mexico., Band-Schmidt CJ; Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas, Ave. Instituto Politécnico Nacional s/n, Col. Playa Palo de Santa Rita, C.P. 23096, La Paz, B.C.S., Mexico., Hernández-Sandoval FE; Centro de Investigaciones Biológicas del Noroeste, Av. Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita Sur, C.P. 23096, La Paz, B.C.S., Mexico., Núñez-Vázquez E; Centro de Investigaciones Biológicas del Noroeste, Av. Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita Sur, C.P. 23096, La Paz, B.C.S., Mexico., Bustillos-Guzmán JJ; Centro de Investigaciones Biológicas del Noroeste, Av. Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita Sur, C.P. 23096, La Paz, B.C.S., Mexico. |
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| Jazyk: | angličtina |
| Zdroj: | Heliyon [Heliyon] 2023 Jun 05; Vol. 9 (6), pp. e17018. Date of Electronic Publication: 2023 Jun 05 (Print Publication: 2023). |
| DOI: | 10.1016/j.heliyon.2023.e17018 |
| Abstrakt: | Harmful algae blooms (HABs) are a conspicuous phenomenon that affect the coastal zone worldwide. Aquaculture industry zones are not excluded from being affected by HAB that cause organism mortality and jeopardize their innocuity due to the contamination by phytotoxins with the concomitant economic losses. Direct ingestion of metabolites from HAB species or organisms contaminated with phycotoxins together with dermal absorption of dissolved metabolites (DM), including toxins, are the two main routes of poisoning. From these poisoning routes, the effect of DM, particularly paralytic shellfish toxins (PST), has been relatively understudied. This intoxication route can be conspicuous and could be involved in many significant mortalities of cultivated marine organisms. In this study, white shrimp juveniles (2.1 g wet weight) of Litopenaeus vannamei were exposed to extracts of 10 4 , 10 5 and 10 6 cells/L of the dinoflagellate Gymnodinium catenatum , a PST producer. The experiment ended after 17 h of exposure when shrimps exposed to 10 6 cells/L extract started to die and the rest of the shrimps, from this and other treatments, did not respond to gentle physical stimulus and their swimming activity was low and erratic. Toxin concentrations were determined using high performance liquid chromatography while qualitative and quantitative histological damages were assessed on the tissues. In general, most toxins were accumulated in the hepatopancreas where more than 90% were found. Other tissues such as intestine, muscle, and gills contained less than 10% of toxins. Compared to the control, the main significative tissue damages were, loss of up to 80% of the nerve cord, 40% of the muscle coverage area, and reduction of the gill lamella width. Also, atrophy in hepatopancreas was observed, manifested by a decrease in the height of B cells, lumen degeneration and thinning of tubules. Some damages were more evident when shrimps were exposed to higher concentrated extracts of G. catenatum , however, not all damages were progressive and proportional to the extract concentration. These data confirm that PST dissolved enter the shrimp, possibly via the gills, and suggest that dissolved metabolites, including PST, may cause tissue damage. Other dissolved metabolites produced by G. catenatum , alone or in synergy, may also be involved. These results also pointed out the importance of dissolved molecules produced for this dinoflagellate and the potential effect on cultured shrimp. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper (© 2023 The Authors.) |
| Databáze: | MEDLINE |
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