Pterostilbene induces apoptosis in hepatocellular carcinoma cells: Biochemical, pathological, and molecular markers.

Autor: Khalil MI; Department of Biological Sciences, Faculty of Science, Beirut Arab University, Lebanon.; Molecular Biology Unit, Zoology Department, Faculty of Science, Alexandria University, Egypt., Agamy AF; Molecular Biology Unit, Zoology Department, Faculty of Science, Alexandria University, Egypt., Elshewemi SS; Zoology Department, Faculty of Science, Alexandria University, Egypt., Sultan AS; Biochemistry Department, Faculty of Science, Alexandria University, Egypt., Abdelmeguid NE; Zoology Department, Faculty of Science, Alexandria University, Egypt.
Jazyk: angličtina
Zdroj: Saudi journal of biological sciences [Saudi J Biol Sci] 2023 Aug; Vol. 30 (8), pp. 103717. Date of Electronic Publication: 2023 Jun 28.
DOI: 10.1016/j.sjbs.2023.103717
Abstrakt: Worldwide, hepatocellular carcinoma (HCC) is considered the sixth most prevalent cancer and ranked third in causes leading to death. Pterostilbene (PTE), a dimethylated analog of resveratrol, is a phytochemical found in fruits such as blueberries and grapes, and is known for its anticancer effect. The current study intended to investigate the effect of PTE on HepG2 cells. Cell viability, colony-forming potential, lipid peroxidation, catalase enzyme (CAT), superoxide dismutase (SOD), and caspase 3 activities, histone release, and expression levels of mTOR, S6K1, p53, and STAT3 proteins were assessed in PTE-treated HepG2 cells. In addition, the cellular and ultrastructural alterations were evaluated by light and transmission electron microscopy. PTE induced a significant reduction in HepG2 viability in a dose-dependent manner (IC 50 of PTE = 74 ± 6 μM), accompanied by a decrease in colony formation potential. PTE-treated cancer cells exhibited a decrease in lipid peroxidation and CAT activity, and an increase in histone release, caspase-3, and SOD activities. Ultrastructurally, PTE-treated cells exhibited notable cell shrinkage, reduced number of filopodia, increased vacuolization, apoptotic bodies, accumulation of lipid droplets, enlarged mitochondria, dilated endoplasmic reticulum, pyknotic nuclei, and cellular fragmentation. mTOR, S6K1, and STAT3 levels were downregulated, however p53 level was modulated in PTE-treated cells. The anticancer potential of PTE might be related to its ability to alter the ultrastructure morphology, reduce mitotic activity, and modulate some key protein required for cell proliferation, suggesting its potential to trigger cancer cells towards apoptosis.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2023 The Author(s).)
Databáze: MEDLINE
Externí odkaz: