Activation of N-Methyl-D-aspartate receptor contributed to the ultrasonic modulation of neurons in vitro.

Autor: Yong W; Department of Physiology, School of Medicine, Jinan University, Guangzhou, China; Department of Biomedical Engineering, Faculty of Engineering, The Hong Kong Polytechnic University, Hong Kong, China., He D; Department of Physiology, School of Medicine, Jinan University, Guangzhou, China., Chen T; Department of Physiology, School of Medicine, Jinan University, Guangzhou, China., Rui Z; Department of Physiology, School of Medicine, Jinan University, Guangzhou, China., Wen Y; Department of Anesthesiology, Shenzhen Maternity & Child Healthcare Hospital, The First School of Clinical Medicine, Southern Medical University, China., Wong KF; Department of Biomedical Engineering, Faculty of Engineering, The Hong Kong Polytechnic University, Hong Kong, China., Sun L; Department of Biomedical Engineering, Faculty of Engineering, The Hong Kong Polytechnic University, Hong Kong, China., Qi X; Department of Anesthesiology, Shenzhen Maternity & Child Healthcare Hospital, The First School of Clinical Medicine, Southern Medical University, China. Electronic address: xiaofeiqi@smu.edu.cn., Guo J; Department of Physiology, School of Medicine, Jinan University, Guangzhou, China; School of Medicine, The Chinese University of Hong Kong, Shenzhen, 518172, Guangdong, China. Electronic address: guojinghui@cuhk.edu.cn.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2023 Oct 08; Vol. 676, pp. 42-47. Date of Electronic Publication: 2023 Jul 17.
DOI: 10.1016/j.bbrc.2023.07.034
Abstrakt: Ultrasound stimulation is increasingly used to investigate brain function and treat brain diseases due to its high level of safety and precise spatiotemporal resolution. Therefore, it is crucial to understand the underlying mechanisms involved in ultrasound brain stimulation. In this study, we investigate the role of NMDA receptors in mediating the effects of ultrasound on primary hippocampal neurons in mice. Our results show that ultrasound alone can activate heterologous NMDA receptor subunits, including NR1A, NR2A, and NR2B, in 293T cells, as well as endogenous NMDA receptors in primary neurons. This activation leads to an influx of calcium and an increase in nuclear c-Fos expression in primary neurons that have not been pre-treated with an NMDA receptor inhibitor. In conclusion, our findings demonstrate that NMDA receptors contribute to neuronal activation by ultrasound stimulation in vitro, providing insight into the molecular mechanisms of ultrasound neuromodulation and a new mediator for the sonogenetics technique.
Competing Interests: Declaration of competing interest All the authors declare that they have no conflict of interest.
(Copyright © 2023. Published by Elsevier Inc.)
Databáze: MEDLINE
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