Effects of simulated microgravity and vibration on osteoblast and osteoclast activity in cultured zebrafish scales.

Autor: Carvajal-Agudelo JD; Department of Biology, Mount Saint Vincent University, Halifax, NS, B3M 2J6, Canada., McNeil A; Department of Biology, Mount Saint Vincent University, Halifax, NS, B3M 2J6, Canada., Franz-Odendaal TA; Department of Biology, Mount Saint Vincent University, Halifax, NS, B3M 2J6, Canada. Electronic address: tamara.franz-odendaal@msvu.ca.
Jazyk: angličtina
Zdroj: Life sciences in space research [Life Sci Space Res (Amst)] 2023 Aug; Vol. 38, pp. 39-45. Date of Electronic Publication: 2023 May 14.
DOI: 10.1016/j.lssr.2023.05.002
Abstrakt: Zebrafish cultured scales have been used effectively to study cellular and molecular responses of bone cells. In order to expose zebrafish scales to simulated microgravity (SMG) and/or vibration, we first determined via apoptosis staining whether cells of the scale survive in culture for two days and hence, we restricted our analyses to two-day durations. Next, we measured the effects of SMG and vibration on cell death, osteoclast tartrate-resistant acid phosphatase, and osteoblast alkaline phosphatase activity and on the number of Runx2a positive cells. We found that during the SMG treatment, osteoclast tartrate-resistant acid phosphatase activity increased on average, while the number of Runx2a positive cells decreased significantly. In contrast, SMG exposure caused a decrease in osteoblast activity. The vibration treatment showed an increase, on average, in the osteoblast alkaline phosphatase activity. This study demonstrates the effect of SMG and vibration on zebrafish scales and the effects of SMG on bone cells. We also show that zebrafish scales can be used to examine the effects of SMG on bone maintenance.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Committee on Space Research (COSPAR). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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